SMARTer Human TCR a/b Profiling Kit—Obtain Full-Length Sequences of TCR-alpha and TCR-beta V(D)J Variable Regions
The SMARTer Human TCR a/b Profiling Kit provides a powerful new solution for those seeking to perform T-cell receptor (TCR) repertoire analysis using NGS. The kit employs a 5’ RACE-like approach to capture complete V(D)J variable regions of TCR transcripts, starting from human blood RNA samples (10 ng–3 µg) or directly from intact lymphocytes (50–10,000 purified T cells). As the name suggests, the kit can be used to generate data for both TCR-alpha and TCR-beta chain diversity, either in the same experiment or separately. In contrast with TCR profiling methods that involve PCR multiplexing, each round of library amplification utilizes a single primer pair for each TCR subunit. Libraries produced with the kit are indexed and ready for sequencing on Illumina® platforms.
This kit leverages our proven SMART technology (Switching Mechanism at 5’ End of RNA Template) along with LNA technology to amplify TCR mRNA sequences in an unbiased manner with remarkable sensitivity.