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cDNA Synthesis & Library Construction


Products >  cDNA_Synthesis_and_Library_Construction >  Next_Gen_Sequencing_Kits >  Single_cell_RNA_Seq_Kits_for_mRNA_seq >  Legacy_Single_Cell_RNA_Seq

Legacy Single-Cell RNA-Seq Kits

Clontech offers multiple kits for single-cell transcriptome analysis. The core SMART (Switching Mechanism at 5’ End of RNA Template) technology in these legacy kits is referred to as SMART-seq™ in the literature, and has shown to be an immensely powerful tool for transcript counting and studying splice junctions and alternative splice variants in cells. These kits use oligo(dT) priming and allow for cDNA synthesis directly from whole cells, without any additional sample preparation, or from total RNA of high integrity (RIN 8–10). cDNA libraries can be simply and efficiently generated for transcriptome analysis (mRNA-seq) from single cells, a few to 1,000 intact cells, or very low amounts of total RNA (10 pg–10 ng).

The legacy SMARTer Ultra Low kits generate libraries that are compatible with Illumina's HiSeq®, or MiSeq® instruments.

For the most advanced cDNA synthesis from single cells we recommend the SMARTer Ultra Low Input RNA Kit for Sequencing - v3.

At-A-Glance Documents Images & Data Resources

Features

  • Sample prep made easySingle-tube protocol that eliminates sample prep by working directly on cells
  • RNA-seq data quality—High mapping; high gene counts; full length gene analysis with no 5' or 3' bias
  • Integration with Illumina sequencing—Compatible with Illumina's NGS platforms

Applications

  • cDNA synthesis and library generation from small quantities of cells or total RNA for transcriptome sequencing using Illumina's Genome Analyzer, HiSeq and MiSeq instruments
  • cDNA output from these kits can be used with either our Low Input Library Prep Kit or with the Nextera® DNA Sample Preparation Kit using our modified protocol

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Package Size Price License # of Units Select
634820 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 12 Rxns $1,560.00 License Statements
634823 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 24 Rxns $2,730.00 License Statements
634826 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 48 Rxns $4,370.00 License Statements
634828 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 96 Rxns $7,010.00 License Statements
634830 SMARTer® Ultra™ Low Input RNA for Illumina® Sequencing - HV 480 Rxns $26,800.00 License Statements
  634936 SMARTer® Ultra™ Low RNA Kit for Illumina® Sequencing 100 Rxns $8,100.00 License Statements
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
639207 Advantage® 2 PCR Kit 30 Rxns $135.00
634947 Low Input Library Prep Kit 12 Rxns $1,140.00
 

 

 

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