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A clinical grade anti-CD3 monoclonal antibody (OKT3) for T cell expansion protocols

The anti-CD3 monoclonal antibody (OKT3 clone) recognizes CD3, an antigen that is expressed on T cells, some NKT cells, and thymocytes during T cell differentiation. OKT3 stimulation is often used in gene therapy protocols to expand T lymphocytes in culture. Manufactured as a quality-assured product according to guidelines for Good Manufacturing Practice (GMP) for Investigational Products, this Anti-CD3 mAb (GMP) can be used for ex vivo applications including T cell expansion.

A complete workflow for generating recombinant adeno-associated viral vectors

Recombinant adeno-associated virus (AAV) vectors are popular tools for gene delivery to mammalian cells and for gene therapy. Clontech now offers a complete workflow for AAV particle production:

  • AAV Packaging—Unique system generates high titers of AAV particles without the use of a helper virus.
  • AAV Particle Extraction—Extraction solution increases particle recovery by ≥3-fold as compared to conventional freeze-thaw methods.
  • AAV Particle Purification—Column-based method for obtaining high purity, high titer AAV particles suitable for in vivo transduction.
  • AAV Titration—Precise quantification using qPCR.

DAB Substrate for Horseradish Peroxidase Detection

TaKaRa DAB Substrate includes all of the reagents needed to prepare DAB substrate for detection of peroxidase-conjugated antibodies used in immunohistochemical staining and Western blotting.

A New Reagent for Immunohistochemical Staining of Paraffin Embedded Tissue Sections

A simpler and easier method to obtain clear, highly sensitive immunohistochemical staining results with minimal background. TaKaRa POD Conjugate replaces traditional secondary antibodies and is available in several formats based on the type of primary antibody for both mouse and mammalian tissue staining.

New ELISA kits

  • Human FGF19 Assay Kit & Human FGF21 Assay Kit: The fibroblast growth factor (FGF) subfamily, is composed of FGF19, FGF21, and FGF23, bind the receptors α/β Klotho, and is characterized as a factor group with hormonal functions. FGF19 is secreted from the intestines and transported to the liver through the bloodstream. FGF21 is secreted in the liver, and reportedly has lipolytic action in fatty tissues. These kits can assay human blood for density of FGF19 and FGF21, respectively.
  • Rat DMP1 Assay Kit: Dentin Matrix Protein 1 (DMP1) is a constitutive protein in the extracellular matrix of osteocytes, and also acts as a regulator of endocrine cells which secrete fibroblast growth factor 23 (FGF-23). This ELISA kit can quantitatively measure the concentration of DMP1 in rat blood.
  • Human α-Synuclein Assay Kit: α-Synuclein is known as an intrinsically disordered protein, involved with the signal regulatory system of synaptic vesicles, and is a main structural component of the Lewy body. This ELISA kit can quantitatively measure its concentration in human blood or human cerebrospinal fluid (CSF).

Choose the best agarose gel for your application

PrimeGel Agarose is available in several different formats for creating agarose gels that are optimized for various nucleic acid fragment size ranges and applications, or for electrophoretic separation of proteins. Specialized PrimeGel products are available for:

  • Separating and/or recovering DNA fragments of less than 1 kb, or up to 20 kb
  • Blotting, pulsed gel electrophoresis, and recovery of DNA fragments for enzymatic reactions such as restriction digestion, ligations, and PCR
  • In-gel enzymatic reactions such as restriction digestion, PCR, or sequencing that can be performed directly in remelted gel made from low-melting agarose, without further purification
  • Immuno-electrophoresis, serum protein electrophoresis, and preparation of agarose beads

Sensitive detection of acrolein in biological and environmental samples

Acrolein is a highly cytotoxic environmental contaminant produced by burning of petroleum, coal, wood, and plastic, and found in cigarette smoke and exhaust gas. The biological and physiological effects of acrolein are being actively investigated. To assist in these efforts, the Acrolein-Lysine Adduct Competitive EIA Kit has been developed.

  • A competitive, single-antibody EIA kit
  • Sensitive, quantitative kit for the detection of acrolein-protein adducts
  • Detect acrolein in blood, urine, or tissue samples as well as environmental samples

Detect mouse feeder cell contamination in stem cell cultures

Mouse feeder cells are used to support the growth and cultivation of stem cells such as hESC (human embryonic stem cells) and hiPSC (human induced pluripotent stem cells)—but they must be removed before use in certain applications, such as regenerative medicine. The Mouse Feeder Cell Quantification Kit is designed to assess stem cell culture quality by measuring mouse feeder cell contamination in genomic DNA extracted from stem cell cultures—using real-time PCR to detect and quantify a mouse mitochondrial (mt) DNA sequence.

  • Detect and quantify residual mouse feeder cells using real-time PCR
  • Get highly sensitive detection of a mouse mtDNA sequence with mtDNA PCR primers
  • Quantify mouse mtDNA copy number by using nuclear DNA PCR primers for normalization
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