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A Complete Workflow for Generating Recombinant Adeno-Associated Viral Vectors

Recombinant adeno-associated virus (AAV) vectors are popular tools for gene delivery to mammalian cells and for gene therapy. Clontech now offers a complete workflow for AAV particle production:

  • AAV Packaging—Unique system generates high titers of AAV particles without the use of a helper virus.
  • AAV Particle Extraction—Extraction solution increases particle recovery by ≥3-fold as compared to conventional freeze-thaw methods.
  • AAV Particle Purification—Column-based method for obtaining high purity, high titer AAV particles suitable for in vivo transduction.
  • AAV Titration—Precise quantification using qPCR.

New Antibodies and ELISA kits

  • Human Lipoprotein Lipase (LPL): Lipoprotein lipase (LPL) is involved in hydrolysis of lipoproteins, including circulating chylomicrons (CMs) and very low-density lipoproteins (VLDLs). This ELISA kit can be used to measure small amounts of circulating LPL in pre-heparin blood samples or LPL levels in cell culture supernatants.
  • Anti-Human G-CSF (4-12-2) Monoclonal Antibody: Granulocyte colony-stimulating factor (G-CSF) controls production, differentiation, and function of granulocytes. Use this affinity-purified monoclonal antibody to detect human G-CSF protein by Western blot or immunohistochemistry.

Clinical Grade RetroNectin® Reagent Now Available

RetroNectin Reagent has been widely used to improve retroviral- and lentiviral- mediated gene transfer into a variety of mammalian cell types. Clinical grade RetroNectin Reagent, for use in ex vivo clinical applications, is now available. RetroNectin (GMP) Reagent is manufactured as a quality-assured product, according to guidelines for Good Manufacturing Practice (GMP) for Investigational Products.

 
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