PrimeScript Reverse Transcriptase

• Complete Elongation: PrimeScript RTase works efficiently on higher-structured RNA templates
• Higher Yield of Full-length cDNA: PrimeScript™ RTase offers high yield transcription with full length cDNA synthesis up to 12 kb
• Fast: can complete reverse transcription in just 15 minutes

CellAmp Whole Transcriptome Amplification Kit (Real Time) Ver.2

• Allows direct amplification of cDNA from a small number of cultured cells (<1,000) using real-time RT-PCR
• Allows real-time PCR (qPCR) analysis of multiple genes from small samples
• Increased amplification yield for genes with low expression levels

siRNA Quantitation Core Kit

• Detects siRNA in total RNA with SYBR Green I real time PCR
• Works with low copy number

EpiTaq HS (for Bisulfite-treated DNA) for DNA Methylation Analysis

• Superior amplification of bisulfite-treated DNA
• Efficient amplification of a range of amplicons, from short (150 bp)  to long (1,000 bp)
• Hot Start enzyme inhibits non specific amplification
• Amplified product can be used directly for TA cloning

EpiScope Nucleosome Preparation Kit

• For preparation of nucleosomal DNA from 0.5 to 2 x 106 mammalian cultured cells
• Enables detailed analysis of nucleosome positioning
• Prepared DNA consists of primarily mononucleosomes

B. subtilis Secretory Protein Expression System

This expression system is amenable to medium- and large-scale fermentation, has a lack of codon bias, and is designated by the U.S. Food and Drug Administration as an organism that is Generally Regarded As Safe (GRAS)

• Includes pBE-S DNA, an E. coli/B. subtilis shuttle vector with B. subtilis-derived subtilisin (aprE) promoter, secretory signal peptide (aprE SP), Multiple Cloning Site, and 3' (C-terminal) His-tag sequence
• Supplied with SP DNA Mixture, a library of DNA sequences encoding 173 unique secretory signal peptides that can be inserted upstream of your target gene
• Fully compatible with In-Fusion cloning kits and systems to allow rapid and easy construct generation
• Includes B. subtilis strain RIK1285

Human Cell Free Expression System

The Human Cell-Free Protein Expression System is an easy to use system for in vitro transcription and translation. The single-tube reaction is easily assembled and protein synthesis is complete in as little as 1 h. This system provides high yield (e.g., 50 µg/ml of human eIF4G) of functional protein, including proteins requiring modifications such as glycosylation, phosphorylation, or fatty acylation and even with large proteins (over 150 kDa).

• Easy-to-use system allows generation of protein in as little as 1 h in a single-tube reaction
• Provides higher yield of functional protein and greater consistency than rabbit reticulocyte or wheat germ in vitro translation systems
• Excellent with challenging proteins such as large proteins (over 150 kDa) and proteins requiring post-translational modification

New Additions to our Line of Highly Sensitive and Quantitative Osteocalcin EIA Kits

Study the role that osteocalcin plays in bone metabolism, insulin synthesis, glucose metabolism, and male fertility. Takara’s Osteocalcin EIA Kits enable quantification of active protein (carboxylated OC for bone metabolism or undercarboxylated OC for regulation of insulin synthesis and glucose metabolism).

• Selection: Choose from kits that quantify the carboxylated or undercarboxylated forms of osteocalcin
• Sensitivity: Measure as little as 0.5 ng/mL of protein
• Convenience: Supplied in a 96 well format (8x12 strips)
• Sandwich format: for highly sensitive quantitative measurement

New Products:

Pig Gla-Osteocalcin EIA Kit

Pig Glu-Osteocalcin EIA Kit

Mouse Gla-Osteocalcin High Sensitive EIA Kit