Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Support
Applications


Support >  Applications >  Restriction_Enzymes >  Quality_Control_Genomic_DNA

Star Activity of Restriction Enzymes

Genome DNA Analysis Grade Restriction Enzyme

Table of genome DNA for QC/Table showing amounts of enzymes required for complete digestion

When cutting various genome DNAs with restriction enzymes for the analysis of the genome DNAs, there is a wide variation of suitable restriction enzymes depending on the genome DNA species.

Takara selects suitable restriction enzymes for various genome DNA analysis, cuts appropriate bacterial genome DNA (agarose embedded; 0.5 µg DNA/50 µL gel) with each lot of the enzymes, and performs pulsed-field electrophoresis to confirm DNA bands pattern.

Amounts of enzymes required for complete digestion of bacterial genome DNA for QC was measured. To measure minimum amounts of enzyme required for complete digestion, 5, 10, 20 and 50 units of each enzyme was added and incubated for 5 and 20 hours.

Table of genome DNA for QCTable showing amounts of enzymes required for complete digestion
Restriction
enzyme
Recognition
sequences*
Genome DNA for QCReaction
buffer**
Reaction
temperature (°C)
Amounts of enzymes required
for complete digestion
Incubation
for 5 hrs
Incubation
for 20 hrs.
Aat II GACGTC Staphylococcus aureus T+BSA 37 50 5
Aor 51H I AGCGCT Staphylococcus aureus M 37 50 20
Bgl I GCCNNNNNGGC Staphylococcus aureus Basal 37 10 5
Bln I CCTAGG Escherichia coli K 37 5 5
BspT104 I TTCGAA Arthrobacter luteus L 37 40 5
BssH II GCGCGC Staphylococcus aureus M 50 5

5

Bst1107 I GTATAC Arthrobacter luteus K 37 5 5
Cla I ATCGAT Arthrobacter luteus M 30 50 20
Cpo I CGGWCCG Staphylococcus aureus K 30 5 5
Dra I TTTAAA Pseudomonas aeruginosa M 37 100*** 100***
Eco52 I CGGCCG Staphylococcus aureus Basal 37 10 5
Mlu I ACGCGT Staphylococcus aureus H 37 5 5
Mun I CAATTG Arthrobacter luteus M+BSA 37 5

5

Nae I GCCGGC Staphylococcus aureus L 37 50 50
Nhe I GCTAGC Arthrobacter luteus M 37 50 20
Not I GCGGCCGC Escherichia coli H+BSA+Tri. 37 5 5
Nru I TCGCGA Staphylococcus aureus Basal 37 50 5
Nsb I TGCGCA Arthrobacter luteus T+BSA 37 5 5
PshB I ATTAAT Pseudomonas aeruginosa Basal 37 5 5
Psp1406 I AACGTT Arthrobacter luteus T+BSA 37 5 5
Pvu I CGATCG Flavobacterium okeanokoites K+BSA 37 20 5
Sac II CCGCGG Staphylococcus aureus T+BSA 37 10 5
Sal I GTCGAC Staphylococcus aureus H 37 150 5
Sfi I GGCCNNNNNGGCC Escherichia coli M 50 100 50
Sma I CCCGGG Staphylococcus aureus T+BSA 30 5 5
Smi I ATTTAAAT Escherichichia coli H 30 50 5
SnaB I TACGTA Arthrobacter luteus Basal 37 10 10
Spe I ACTAGT Escherichia coli M 37 50 20
Sse8387 I CCTGCAGG Staphylococcus aureus M+BSA 37 5 5
Ssp I AATATT Arthrobacter luteus Basal 37 5 5
Xba I TCTAGA Escherichia coli M+BSA 37 50 5
Xho I CTCGAG Escherichia coli H 37 20 5

* W: A or T, N: A or C or G or T
** 0.5 µg DNA/50 µL gel+100 µL reaction mixture
*** Reaction of Dra I is extremely slow in agarose gel. Thus optimal amounts were added and left at 4°C for 16 hours. The samples were then transferred to 37°C and incubated for 5 or 20 hours.

References

  1. McClelland, M. (1987) Gene Amplification and Analysis 5 (Chirikijan, J. G. ed.), 257-282.2.
  2. McClelland, M., Patel, Y. and Nelson, M. (1987) Nucleic Acids Res., 15, 5985-6005.
  3. Daniels, D. L. (1990) Nucleic Acids Res., 18, 2649-2651.

« Back to Restriction Enzyme Technical Information

^ to top

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions