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Support >  Applications >  Restriction_Enzymes >  Inactivation

Residual Enzyme Activity Following Inactivation Treatment

Residual Enzyme Activity Following Inactivation Treatment

Heat treatment is generally applied to inactivate restriction enzymes after completion of a reaction. Heat tolerance varies depending on the kind of enzyme; however, in some cases heat treatment is insufficient to inactivate restriction enzymes completely. Therefore, four kinds of inactivation treatment were used for each enzyme, and the residual enzyme activity was measured to determine the best conditions for complete inactivation.

Restriction
Enzyme
60°C
15 min.
70°C
15 min.
Ethanol
Precipitation
Phenol
Extraction
  Restriction
Enzyme
60°C
15 min.
70°C
15 min.
Ethanol
Precipitation
Phenol
Extraction
Aat II+ -- --NT   Hha I + -- -- NT
Acc I+ -- --NT   Hinc II + -- -- NT
Acc II++ --NT   Hind III + -- -- NT
Acc IIINT -- --NT   Hinf I + -- -- NT
Afa I + -- + --   Hin1 I -- NT + --
Afl II -- NT -- NT   Hpa I -- NT -- NT
Alu I --NT+ --   Kpn I -- NT -- NT
Aor13H INT++ --   Mbo I + -- -- NT
Aor51H I --NT --NT   Mbo II + -- -- NT
Apa I --NT --NT   Mfl I + + + --
ApaL I+ -- -- NT   Mlu I + + -- NT
Ava II+ --+ --   Msp I -- NT + --
Avi II+ --+ --   Mun I + -- + --
Bal I -- NT + --   Nae I -- NT -- NT
BamH I -- NT + NT   Nco I + -- + --
Ban II -- NT -- NT   Nde I + -- -- NT
Bcn I + + -- NT   Nhe I + -- -- NT
Bgl I -- NT + --   Not I + + + --
Bgl II + -- + --   Nru I -- NT + --
Bln I + + -- NT   PmaC I -- NT -- NT
Bpu1102 I + + + --   PshA I -- NT -- NT
Bsp1286 I -- NT -- NT   PshB I + -- -- NT
Bsp1407 I + -- + --   Psp1406 I -- NT + --
BspT107 I -- NT -- NT   Pvu I -- NT -- NT
BssH II + -- -- NT   Pvu II + + + --
BstP I NT + -- NT   Sac I -- NT -- NT
BstX I -- NT -- NT   Sac II + -- + --
Bst1107 I + + + --   Sal I -- NT -- NT
Cfr10 I + + + --   Sau3A I + -- -- NT
Cfr13 I -- NT -- NT   Sca I -- NT -- NT
Cla I -- NT -- NT   Sfi I + -- + --
Cpo I -- NT -- NT   Sma I -- NT + --
Dra I -- NT -- NT   SnaB I -- NT -- NT
Dde I + -- -- --   Spe I -- NT -- NT
Eae I -- NT -- NT   Sph I -- NT -- NT
Eam1105 I -- NT + --   Sse8387 I -- NT -- NT
EcoO109 I -- NT -- NT   Ssp I -- NT -- NT
EcoO65 I + -- + --   Stu I -- NT + NT
EcoR I -- NT -- NT   Taq I NT + + --
EcoR V + -- + --   Tth 111 I NT + -- NT
EcoT14 I -- NT -- NT   Van91 I -- NT + --
EcoT22 I -- NT -- NT   VpaK11B I -- NT -- NT
Eco52 I -- NT + --   Xba I -- NT + --
Eco81 I + -- -- NT   Xho I + + -- NT
Fba I + + -- NT   Xsp I + + + --
Fok I -- NT + --            
Fse I -- NT -- NT            
Hae II -- NT -- NT            
Hae III + -- -- NT            
Hap II + -- + --            

+ : Residual activity remaining // -- : No residual activity // NT : Not tested

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