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Support >
Applications >
Restriction_Enzymes >
Inactivation
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Residual Enzyme Activity Following Inactivation Treatment
Residual Enzyme Activity Following Inactivation Treatment
Heat treatment is generally applied to inactivate restriction enzymes after completion of a reaction. Heat tolerance varies depending on the kind of enzyme; however, in some cases heat treatment is insufficient to inactivate restriction enzymes completely. Therefore, four kinds of inactivation treatment were used for each enzyme, and the residual enzyme activity was measured to determine the best conditions for complete inactivation.
Restriction
Enzyme
| 60°C
15 min. | 70°C
15 min. | Ethanol
Precipitation | Phenol
Extraction |
|
Restriction
Enzyme |
60°C
15 min. |
70°C
15 min. |
Ethanol
Precipitation |
Phenol
Extraction |
| Aat II | + |
-- |
-- | NT |
|
Hha I |
+ |
-- |
-- |
NT |
| Acc I | + |
-- |
-- | NT |
|
Hinc II |
+ |
-- |
-- |
NT |
| Acc II | + | + |
-- | NT |
|
Hind III |
+ |
-- |
-- |
NT |
| Acc III | NT |
-- |
-- | NT |
|
Hinf I |
+ |
-- |
-- |
NT |
| Afa I |
+ |
-- |
+ |
-- |
|
Hin1 I |
-- |
NT |
+ |
-- |
| Afl II |
-- |
NT |
-- |
NT |
|
Hpa I |
-- |
NT |
-- |
NT |
| Alu I |
-- | NT | + |
-- |
|
Kpn I |
-- |
NT |
-- |
NT |
| Aor13H I | NT | + | + |
-- |
|
Mbo I |
+ |
-- |
-- |
NT |
| Aor51H I |
-- | NT |
-- | NT |
|
Mbo II |
+ |
-- |
-- |
NT |
| Apa I |
-- | NT |
-- | NT |
|
Mfl I |
+ |
+ |
+ |
-- |
| ApaL I | + |
-- |
-- |
NT |
|
Mlu I |
+ |
+ |
-- |
NT |
| Ava II | + |
-- | + |
-- |
|
Msp I |
-- |
NT |
+ |
-- |
| Avi II | + |
-- | + |
-- |
|
Mun I |
+ |
-- |
+ |
-- |
| Bal I |
-- |
NT |
+ |
-- |
|
Nae I |
-- |
NT |
-- |
NT |
| BamH I |
-- |
NT |
+ |
NT |
|
Nco I |
+ |
-- |
+ |
-- |
| Ban II |
-- |
NT |
-- |
NT |
|
Nde I |
+ |
-- |
-- |
NT |
| Bcn I |
+ |
+ |
-- |
NT |
|
Nhe I |
+ |
-- |
-- |
NT |
| Bgl I |
-- |
NT |
+ |
-- |
|
Not I |
+ |
+ |
+ |
-- |
| Bgl II |
+ |
-- |
+ |
-- |
|
Nru I |
-- |
NT |
+ |
-- |
| Bln I |
+ |
+ |
-- |
NT |
|
PmaC I |
-- |
NT |
-- |
NT |
| Bpu1102 I |
+ |
+ |
+ |
-- |
|
PshA I |
-- |
NT |
-- |
NT |
| Bsp1286 I |
-- |
NT |
-- |
NT |
|
PshB I |
+ |
-- |
-- |
NT |
| Bsp1407 I |
+ |
-- |
+ |
-- |
|
Psp1406 I |
-- |
NT |
+ |
-- |
| BspT107 I |
-- |
NT |
-- |
NT |
|
Pvu I |
-- |
NT |
-- |
NT |
| BssH II |
+ |
-- |
-- |
NT |
|
Pvu II |
+ |
+ |
+ |
-- |
| BstP I |
NT |
+ |
-- |
NT |
|
Sac I |
-- |
NT |
-- |
NT |
| BstX I |
-- |
NT |
-- |
NT |
|
Sac II |
+ |
-- |
+ |
-- |
| Bst1107 I |
+ |
+ |
+ |
-- |
|
Sal I |
-- |
NT |
-- |
NT |
| Cfr10 I |
+ |
+ |
+ |
-- |
|
Sau3A I |
+ |
-- |
-- |
NT |
| Cfr13 I |
-- |
NT |
-- |
NT |
|
Sca I |
-- |
NT |
-- |
NT |
| Cla I |
-- |
NT |
-- |
NT |
|
Sfi I |
+ |
-- |
+ |
-- |
| Cpo I |
-- |
NT |
-- |
NT |
|
Sma I |
-- |
NT |
+ |
-- |
| Dra I |
-- |
NT |
-- |
NT |
|
SnaB I |
-- |
NT |
-- |
NT |
| Eae I |
-- |
NT |
-- |
NT |
|
Spe I |
-- |
NT |
-- |
NT |
| Eam1105 I |
-- |
NT |
+ |
-- |
|
Sph I |
-- |
NT |
-- |
NT |
| EcoO109 I |
-- |
NT |
-- |
NT |
|
Sse8387 I |
-- |
NT |
-- |
NT |
| EcoO65 I |
+ |
-- |
+ |
-- |
|
Ssp I |
-- |
NT |
-- |
NT |
| EcoR I |
-- |
NT |
-- |
NT |
|
Stu I |
-- |
NT |
+ |
-- |
| EcoR V |
+ |
-- |
+ |
-- |
|
Taq I |
NT |
+ |
+ |
-- |
| EcoT14 I |
-- |
NT |
-- |
NT |
|
Tth 111 I |
NT |
+ |
-- |
NT |
| EcoT22 I |
-- |
NT |
-- |
NT |
|
Van91 I |
-- |
NT |
+ |
-- |
| Eco52 I |
-- |
NT |
+ |
-- |
|
VpaK11B I |
-- |
NT |
-- |
NT |
| Eco81 I |
+ |
-- |
-- |
NT |
|
Xba I |
-- |
NT |
+ |
-- |
| Fba I |
+ |
+ |
-- |
NT |
|
Xho I |
+ |
+ |
-- |
NT |
| Fok I |
-- |
NT |
+ |
-- |
|
Xsp I |
+ |
+ |
+ |
-- |
| Fse I |
-- |
NT |
-- |
NT |
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| Hae II |
-- |
NT |
-- |
NT |
|
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| Hae III |
+ |
-- |
-- |
NT |
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| Hap II |
+ |
-- |
+ |
-- |
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+ : Residual activity remaining // -- : No residual activity // NT : Not tested
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