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Products >  Real_Time_qPCR_Kits >  SYBR_Detection >  SYBR_Premix_Ex_Taq_Tli_RNaseH_Plus

SYBR qPCR: SYBR  Premix Ex Taq (Tli RNase H Plus)

The Takara Bio SYBR Premix Ex Taq (Tli RNase H Plus), a SYBR qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies. SYBR Premix Ex Taq (Tli RNase H Plus) includes SYBR Green I, a qPCR reagent  for intercalator-based RT-PCR (qPCR). In addition to being ideal for most standard real-time PCR reactions, this kit is recommended when a higher yield of product is needed or when amplifying longer products (up to 570 bp). This SYBR qPCR premix is supplied at a 2X concentration and allows easy reaction assembly; by simply adding primers, template and sterile distilled water, intercalator-based qPCR can be performed.

SYBR Premix Ex Taq (Tli RNase H Plus) contains TaKaRa Ex Taq HS, a hot start PCR enzyme that includes an anti-Taq antibody. The TaKaRa Ex Taq HS enzyme, in combination with its qPCR optimized buffer, allows for high amplification efficiency and detection sensitivity in qPCR. The 2X SYBR qPCR premix also contains Tli RNase H, a heat-resistant RNase H that minimizes qPCR inhibition as a consequence of residual mRNA in the input template cDNA. This product enables accurate detection of targets even during high-speed reactions, making it possible to conduct fast RT-PCR (qPCR) analyses and achieve excellent reproducibility and reliability.

ROX Reference Dyes are supplied as separate components with Cat.# RR420A, RR420B, RR420L and RR420W. These reference dyes serve as convenient internal reference standards when normalizing non-PCR generated fluorescence fluctuations; such fluctuations often occur between different wells or over time in different instruments.

Cat.# RR420L and RR420W are larger package sizes (5 mL and 25 mL (5 x 5 mL, respectively). Each provide convenient larger packaging and are supplied with a single, separate vial of ROX Reference Dye.

Cat.# RR42LR and RR42WR are larger package sizes (5 mL and 25 mL (5 x 5 mL, respectively), that contain ROX Reference Dye in the premix.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Lower Cq values as a result of fast extension rates
  • Use with broad range of amplicon sizes, even over 500 bp
  • Convenient 2X master mix is excellent for high-speed qPCR
  • High sensitivity: detects fewer than 100 copies
  • Hot start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
  • Compatible with all commonly used real-time PCR (qPCR) instruments
  • Minimizes qPCR inhibition due to residual mRNA in input cDNA
  • Includes SYBR Green I for intercalator-based qPCR

Applications

  • SYBR qPCR using SYBR Green I and ROX Reference Dye
  • Gene expression analysis
  • Genotyping by real-time PCR
  • High-throughput real-time PCR
  • Amplification of long products
  • When high yield is critical (e.g., during gene expression analysis of rare cDNAs)

Components

RR420A (200 reactions (50 µL rxns); 500 reactions (20µL rxns))

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)1
1 mL × 5
ROX Reference Dye (50X conc.)2200 µL
ROX Reference Dye II (50X conc.)2200 µL

1: Contains TaKaRa Ex Taq HS DNA Polymerase, dNTP Mixture, Mg2+, Tli RNase H and SYBR Green I

2: This component is to be used with devices that corrects fluorescent signals between wells, such as the real-time PCR device by Applied Biosystems. Please use ROX Reference Dye for Applied Biosystems 7900HT/7300 Real-Time PCR System or StepOnePlus and ROX Reference Dye II for 7500 Real-Time PCR System or 7500 Fast Real-Time PCR System. ROX Reference Dye II is compatible with Agilent Mx3000P. This component is not required with Thermal Cycler Dice Real Time System II, Smart Cycler System or LightCycler.

RR420L (200 reactions (50 µL rxns); 500 reactions (20µL rxns))

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)*1 x 5 mL
ROX Reference Dye (50X conc.) 200 µL
*Contains TaKaRa Ex Taq HS DNA Polymerase, Tli RNase H, dNTP mix, Mg2+ and SYBR Green I

RR420W (1000 reactions (50 µL rxns); 2500 reactions (20µL rxns))

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)*5 x 5 mL
ROX Reference Dye (50X conc.) 1 mL

*Contains TaKaRa Ex Taq HS DNA Polymerase, Tli RNase H, dNTP mix, Mg2+ and SYBR Green I

RR42LR (200 reactions (50 µL rxns); 500 reactions (20µL rxns))

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)*1 x 5 mL

*Contains TaKaRa Ex Taq HS DNA Polymerase, dNTP Mix, Tli RNase H, Mg2+ and SYBR Green I and ROX Reference dye.

RR42WR (1000 reactions (50 µL rxns); 2500 reactions (20µL rxns))

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)*5 x 5 mL

*Contains TaKaRa Ex Taq HS DNA Polymerase, dNTP Mix, Mg2+ and SYBR Green I and ROX Reference dye.

Storage

4°C

Note: Product is stable for up to 6 months when stored at 4°C. Protect from light and avoid contamination. This kit is shipped in a dry ice-packed container. For long-term storage, keep item at –80°C. Do not store at –20°C. Store thawed product at 4°C and use within 6 months.

PCR Performance Test

Consistent amplification results were achieved via SYBR Green I detection of RT-PCR products using a 300 bp lambda DNA template and a Smart Cycler real-time PCR instrument.

References

1. Kim, D.N. et al. (2013) J. Gen. Virol. 94:497-506.

2. Kotani, M. et al. (2013) J. Immunol. 190:605-612.

3. Liu, H. et al. (2012) Drug Metab. Dispos. 40:2109-2118.




 
 
Products
Cat. # Product Contents Size Price License Units Select
RR420A SYBR® Premix Ex Taq™ (Tli RNase H Plus) 200 Rxns $290.00 License Statements
RR420B SYBR® Premix Ex Taq™ (Tli RNase H Plus) 400 Rxns $566.00 License Statements
RR420L SYBR® Premix Ex Taq™ (Tli RNase H Plus), 1 x 5 mL 200 Rxns $290.00 License Statements
RR42LR SYBR® Premix Ex Taq™ (Tli RNase H Plus), 1 x 5 mL, ROX Plus 200 Rxns $290.00 License Statements
RR420W SYBR® Premix Ex Taq™ (Tli RNase H Plus), 5 x 5 mL 1,000 Rxns $1,159.00 License Statements
RR42WR SYBR® Premix Ex Taq™ (Tli RNase H Plus), 5 x 5 mL, ROX Plus 1,000 Rxns $1,159.00 License Statements
 

 

 
 

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