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Products >  Real_Time_qPCR_Kits >  SYBR_Detection >  SYBR_Premix_Ex_Taq_II_Tli_Rnase_H_Plus

SYBR Green Master Mix: SYBR Premix Ex Taq II (Tli RNase H Plus)

This Takara Bio SYBR Green Master Mix is recommended for real-time PCR (qPCR) when high specificity is critical, because this reagent effectively suppresses amplification of primer-dimers and off-target products. Use this product when primer design has been optimized, but an increase in specificity of the qPCR assay is still needed. SYBR Premix Ex Taq II (Tli RNase H Plus) includes SYBR Green I, a reagent designed for intercalator-based qPCR. Additionally, SYBR Premix Ex Taq II (Tli RNase H Plus) is supplied at a 2X concentration, enabling easy reaction assembly. The premix also contains heat-resistant Tli RNase H Plus, which effectively degrades residual mRNA found in the input cDNA, thereby minimizing qPCR inhibition as a result of residual mRNA presence.

Takara SYBR Green Master Mix products contain TaKaRa Ex Taq HS DNA Polymerase, a hot start PCR enzyme that includes an anti-Taq antibody. The use of an anti-Taq antibody allows highly reproducible and reliable qPCR. SYBR Premix Ex Taq II (Tli RNase H Plus) also enables high reaction specificity and superior performance with GC-rich templates.

Two ROX reference dyes are supplied as separate components with Cat. #RR820A, RR820B, RR820L and RR820W. These reference dyes serve as convenient internal reference standards when normalizing non-PCR generated fluorescence fluctuations; such fluctuations often occur between different wells or over time in different instruments.

Cat. #RR820L and RR820W are larger package sizes (5 mL and 25 mL (5 x 5 mL, respectively) of SYBR Premix Ex Taq II (Tli RNase H Plus). Each of these includes a separate vial of ROX Reference Dye.

Cat. #RR82LR and RR82WR are larger package sizes (5 mL and 25 mL (5 x 5 mL, respectively) with ROX Reference Dye included in the premix.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • High specificity minimizes primer-dimer formation and nonspecific amplification
  • Excels with GC-rich targets
  • 2X master mix provides convenience
  • High sensitivity: detects fewer than 100 copies
  • Compatible with all commonly used qPCR instruments
  • Includes SYBR Green Master Mix for intercalator-based qPCR
  • Tli RNase H Plus reduces inhibition from mRNA/cDNA hybrids in qPCR
  • Eliminates need for separate RNase H digestion step when using low RNase H RTs

Applications

  • Gene expression analysis
  • qPCR
  • Genotyping
  • Suppression of primer-dimers or off-target products

Components

RR820A (200 reactions (50 µL rxns); 500 reactions (20 µL rxns))

SYBR Premix Ex Taq II (Tli RNase H Plus) (2X conc.)11 mL × 5
ROX Reference Dye (50X conc.)2200 µL
ROX Reference Dye II (50X conc.)2200 µL

1: Contains TaKaRa Ex Taq HS, dNTP Mixture, Mg2+, Tli RNaseH and SYBR Green I

2: This component is to be used with devices that correct fluorescent signals between wells, such as the real-time PCR device by Applied Biosystems. Please use ROX Reference Dye for Applied Biosystems 7300 Real-Time PCR System or StepOnePlus and ROX Reference Dye II for the 7500/7500 Fast Real-Time PCR System. This component is not required with the Thermal Cycler Dice Real Time System II or LightCycler.

RR820L (200 reactions (50 µL rxns); 500 reactions (20 µL rxns))

SYBR Premix Ex Taq II (Tli RNase H Plus) (2X conc.)*1 x 5 mL
ROX Reference Dye (50X conc.)200 µL

*Contains TaKaRa Ex Taq HS DNA Polymerase, Tli RNase H, dNTP Mix, Mg2+ and SYBR Green I.

RR820W (1000 reactions (50 µL rxns); 2500 reactions (20 µL rxns))

SYBR Premix Ex Taq II (Tli RNase H Plus) (2X conc.)*5 x 5 mL
ROX Reference Dye (50X conc.)1 mL

*Contains TaKaRa Ex Taq HS DNA Polymerase, dNTP Mix, Mg2+ and SYBR Green I.

RR82LR (200 reactions (50 µL rxns) 500 reactions (20 µL rxns))

SYBR Premix Ex Taq II (2X conc.)*1 x 5 mL

*Contains TaKaRa Ex Taq HS DNA Polymerase, Tli RNase H Plus, dNTP Mix, Mg2+, SYBR Green I and ROX dye.

RR82WR (1000 reactions (50 µL rxns); 2500 reactions (20 µL rxns))

SYBR Premix Ex Taq II (Tli RNase H Plus) (2X conc.)*5 x 5 mL


*Contains TaKaRa Ex Taq HS DNA Polymerase, Tli RNase H Plus, dNTP Mix, Mg2+ , SYBR Green I and ROX dye.

Storage

4°C

Note: Product is stable for up to 6 months when stored at 4ºC. Protect this kit from light and avoid contamination. This kit is shipped in a package containing dry ice. For long-term storage, store at –80ºC. Do not store at –20ºC. Store thawed product at 4ºC and use within 6 months.

PCR Performance Test

Consistent amplification results were confirmed by SYBR Green I detection of PCR products in real time using a 300 bp lambda DNA template and a SmartCycler real time PCR instrument.

References

1. Hong, S. et al. (2010) Proc. Natl. Acad. Sci. USA 107:21211-21216.

2. Ishikawa, M. et al. (2011) Invest. Ophthalmol. Vis. Sci. 52:6604-6616.




 
 
Products
Cat. # Product Contents Size Price License Units Select
RR820A SYBR® Premix Ex Taq™ II (Tli RNase H Plus) 200 Rxns $290.00 License Statements
RR820B SYBR® Premix Ex Taq™ II (Tli RNase H Plus) 400 Rxns $566.00 License Statements
RR820L SYBR® Premix Ex Taq™ II (Tli RNase H Plus), 1 x 5 mL 200 Rxns $290.00 License Statements
RR82LR SYBR® Premix Ex Taq™ II (Tli RNase H Plus), 1 x 5 mL, ROX Plus 200 Rxns $290.00 License Statements
RR820W SYBR® Premix Ex Taq™ II (Tli RNase H Plus), 5 x 5 mL 1,000 Rxns $1,159.00 License Statements
RR82WR SYBR® Premix Ex Taq™ II (Tli RNase H Plus), 5 x 5 mL, ROX Plus 1,000 Rxns $1,159.00 License Statements
 

 

 
 

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