Takara
login | register | order now | view cart  
Products
Real Time qPCR Kits
qPCR Premixes
Dilution Solution
Primer Set
Probe Detection
RNA Extraction and Protein Analysis for qPCR
RT-qPCR Reagents
SYBR Detection


Products >  Real_Time_qPCR_Kits >  SYBR_Detection >  One_Step_SYBR_Ex_Taq_qRT-PCR_Kit

One Step SYBR Ex Taq qRT-PCR Kit

One Step SYBR Ex Taq RT-PCR Kit is designed for real time, one-step RT-PCR using SYBR Green I1,2 detection and is suitable for detection of small amounts of RNA (i.e., viral RNA). In this kit, RT-PCR can be performed in a single tube, minimizing contamination. Also, amplified products are monitored in real time, so there is no need to verify results through electrophoresis after PCR.

This kit uses a new RTase, a robust reverse transcriptase which can quickly and efficiently synthesize cDNA and TaKaRa's Ex Taq HS, a high efficiency hot start PCR enzyme. These enzymes have been optimized for One Step qRT-PCR.

Note: This product is available in a sample size.

Compatible Real Time PCR Instruments

Thermal Cycler Dice Real Time System (TP800), ABI PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System (Applied Biosystems), LightCycler (Roche Diaganostics), Smart Cycler System3/Smart Cycler II System3 (Cepheid), and others.

1. SYBR Green I is licensed by Molecular Probes Inc. for research reagents. SYBR is a registered trademark of Molecular Probes Inc.
2. Use One Step Ex Taq RT-PCR Kit (Perfect Real Time) for real time one-step RT-PCR using TaqMan probe for detection. TaqMan is a registered trademark of Roche Molecular Systems Inc.
3. Smart Cycler is a registered trademark of Cepheid.

At-A-Glance Documents Resources

Features

  • Efficient: New RT with strong elongation activity and high-performance TaKaRa Ex Taq Hot Start Version
  • High Specificity: Optimized buffer system and antibody mediated hot start.
  • Superior Sensitivity: Accurate quantification of low quantity samples such as RNA viruses
  • Simple Protocol: One step qRT-PCR reduces amount of pipetting and lowers risk of contamination

Applications

  • One-Step qRT-PCR using SYBR Green Reaction

Components

TAK RR067A (reaction size: 25 µL)
2X One Step SYBR RT-PCR Buffer* 3 x 840 µL
TaKaRa Ex Taq HS (5U/µL) 100 µL
RT Enzyme Mix II 100 µL
RNase Free dH2O 2 x 1.25 mL
ROX Reference Dye (50X conc.)** 100 µL
ROX Reference Dye II (50X conc.)** 100 µL

* Includes dNTP Mixture, Mg2+ and SYBR Green I
** ROX Reference Dye/Dye II is used for normalization of fluorescence intensity by background subtraction. For ABI PRISM 7000/7700/7900HT and Applied Biosystems 7300 Real-Time PCR System, the use of ROX Reference Dye (50X) is recommended. For Applied Biosystems 7500 Real-Time PCR System, the use of ROX Reference Dye II is recommended. The use of ROX Reference Dye or Dye II is optional. It is not required for use with Thermal Cycler Dice Real Time System, Smart Cycler or LightCycler real time instruments.

Storage

–20°C. 2X One Step SYBR RT-PCR Buffer should be protected from light.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR067A One Step SYBR® Ex Taq™ qRT-PCR Kit 100 Rxns $286.00 License Statements
RR067B One Step SYBR® Ex Taq™ qRT-PCR Kit 500 Rxns $1,286.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
3732 CellAmp™ Direct RNA Prep Kit for RT-PCR (Real Time) 200 Rxns $187.00
 

 


Clontech is a Takara Bio Company © 2012 Privacy Policy | Terms & Conditions | TRUSTe