Takara
login | register | order now | view cart  
Products
Real Time qPCR Kits
qPCR Premixes
Dilution Solution
Primer Set
Probe Detection
RNA Extraction and Protein Analysis for qPCR
RT-qPCR Reagents
SYBR Detection


Products >  Real_Time_qPCR_Kits >  Primer_Set >  Transgene_Detection_Primer_Set_for_Real_Time

Transgene Detection Primer Set for Real Time (Mouse)

This kit is a primer set for real-time PCR to detect GFP (EGFP, AcGFP1) and lacZ, which are widely used as an inserted gene marker in transgenic mice. It is possible to screen transgenic mice by detecting these marker genes with real-time PCR.

The primer sets (Ywhaz, Raver2) for detecting genes on mouse genomic DNA are also included as a reference. Therefore, it is possible to make a comparison of the transgene contents between samples by relative quantification method.
At-A-Glance Documents Images & Data

Applications

  • Detection and quantification of transgene inserted in transgenic mouse genome

Components

(for 100 reactionsA in each primer)

ProductConc.AmountSize
GFP primer-1B2 µM each500 µL127 bp
GFP primer-2C2 µM each500 µL162 bp
lacZ primer-1D2 µM each500 µL96 bp
lacZ primer-2D2 µM each500 µL141 bp
Reference primer-1E2 µM each500 µL115 bp
Reference primer-2F2 µM each500 µL89 bp
AFrequency when using SYBR Premix Ex Taq II with 25 µL reaction volume.
BGFP primer-1 is designed for consensus sequence with EGFP and AcGFP1.
CGFP primer-2 is designed for EGFP. It is not used for AcGFP1 detection.
DlacZ primer-1 and -2 are designed for lacZ (beta-galactosidase) gene and positioned at different sites.
EReference primer-1 is for a part of Ywhaz gene on mouse chromosome 15.
FReference primer-2 is for a part of Raver2 gene on mouse chromosome 4.

Storage

–20°C


 
 
Products
Cat. # Product Package Size Price # of Units Select
3788 Transgene Detection Primer Set for Real Time (Mouse) 100 Rxns $187.00
 

 


Clontech is a Takara Bio Company © 2012 Privacy Policy | Terms & Conditions | TRUSTe