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Products >  Real_Time_qPCR_Kits >  Primer_Set >  PrimerArray_Series_Disease_Pathways

RT-PCR Primers: PrimerArray Series for Disease Pathways

When performing gene expression analysis by real-time PCR, ready-to use primer sets for RT-PCR save time, minimize pipetting, and reduce error. The PrimerArray Series are sets of real-time RT-PCR primers for the analysis of the expression of genes associated with specific disease states in human (Cat.# PH011-PH015) or mouse (Cat.# PN011-PN015). These PrimerArray RT-PCR primer sets allow efficient workflows for colorectal cancer gene expression analysis as well as other diseases such as pancreatic cancer, prostate cancer, melanoma, small lung cell cancer, non-small lung cell cancer, asthma, rheumatoid arthritis, and Type I or Type II diabetes.

Each array contains 96 primer pairs representing 88 genes associated with a disease pathway and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. The expression levels of multiple genes can be screened simultaneously. The PrimerArray Analysis Tool Ver. 2.2, available for download on this web page, and/or Multiplate RQ (for use with the Thermal Cycler Dice Real Time Systems, not available in all geographic locations) are useful for analyzing data obtained using these products. Primer set information and the PrimerArray analysis tool can be downloaded from this page (click on the Resources tab, then expand the drop-down menu under Technical Notes).

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Affordable: Each PrimerArray Set supplies sufficient qPCR primers for up to 12 real-time PCR runs (25 µL qPCR reaction volume)
  • Minimizes pipetting and reduces error
  • Enables high-throughput analysis of disease pathway gene expression
  • Optimized for superior performance with SYBR Ex Taq qPCR kits
  • PrimerArray Analysis Tool makes data analysis easy
  • Compatible with all standard real-time PCR instrument systems

Applications

  • Disease pathway gene expression analysis for major diseases such as colorectal cancer, diabetes, melanoma, prostate cancer, lung cancer, rheumatoid arthritis, and asthma
  • Screening for expression of disease pathways in response to drug targets or conditions

Procedural Overview for Gene Expression Analysis by Real Time RT-PCR

The overall workflow for the PrimerArray Sets is straightforward:

  1. RNA extraction (30 min): We recommend NucleoSpin RNA II kits (Clontech Cat.#740955.50) or RNAiso (Cat.# 9108, not available in all geographic locations.)
  2. Reverse transcription (20 min): We recommend PrimeScript RT Master Mix (Perfect Real Time) (Cat.# RR036A)
  3. Dispense real-time PCR reaction solution (10 min): Prepare a master mix of your cDNA sample and SYBR Premix Ex Taq II (Tli RNase Plus) (Cat.# RR820A) and dispense into a 96-well plate.
  4. Add PrimerArray primers (5 min): Use 4 µL per reaction
  5. Real-time PCR (1 - 2 hours): Compatible with all commonly used real-time PCR systems
  6. Data analysis (30 minutes): PrimerArray Analysis Tool makes data analysis easy

PrimerArray Analysis Tool

The PrimerArray analysis tool facilitates data analysis and can be downloaded from this page (click on the Resources tab, then expand the drop-down menu under Technical Notes). For the PrimerArray Analysis Tool v2.2 User Manual, click on the Documents tab, then expand the drop-down menu under User Manuals.

 

Primer Information for RT-PCR Primer Sets

To download primer information for each PrimerArray RT-PCR primer set, click on the Resources tab, then expand the drop-down menu under Technical Notes.




 
 
Products
Cat. # Product Contents Size Price Units Select
PH011 PrimerArray® Colorectal Cancer & Pancreatic Cancer (Human) 1 Set $519.00
PH012 PrimerArray® Prostate Cancer & Melanoma (Human) 1 Set $519.00
PH013 PrimerArray® Small Cell Lung Cancer & Non-small Lung Cancer (Human) 1 Set $519.00
PH014 PrimerArray® Asthma & Rheumatoid Arthritis (Human) 1 Set $519.00
PH015 PrimerArray® Diabetes Mellitus, Type I & Type II (Human) 1 Set $519.00
PN011 PrimerArray® Colorectal Cancer & Pancreatic Cancer (Mouse) 1 Set $519.00
PN012 PrimerArray® Prostate Cancer & Melanoma (Mouse) 1 Set $519.00
PN013 PrimerArray® Small Cell Lung Cancer & Non-small Lung Cancer (Mouse) 1 Set $519.00
PN014 PrimerArray® Asthma & Rheumatoid Arthritis (Mouse) 1 Set $519.00
PN015 PrimerArray® Diabetes Mellitus, Type I & Type II (Mouse) 1 Set $519.00
 

 

Recommended Products
Cat. # Product Contents Size Price License Units Select
RR820A SYBR® Premix Ex Taq™ II (Tli RNase H Plus) 200 Rxns $290.00 License Statements
RR820B SYBR® Premix Ex Taq™ II (Tli RNase H Plus) 400 Rxns $566.00 License Statements
RR036A PrimeScript™ RT Master Mix (Perfect Real Time) 200 Rxns $425.00  
RR036B PrimeScript™ RT Master Mix (Perfect Real Time) 800 Rxns $1,365.00  
 

 

 
 

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