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Products >  Real_Time_qPCR_Kits >  Primer_Set >  PrimerArray_Series

PrimerArray Series

Takara's PrimerArray Series offers primer sets for real time RT-PCR (qPCR) that can be used for gene expression analysis of biological pathways. Each PrimerArray Series primer set contains 96 total primers that allow for the simultaneous analysis of 88 biological pathway-related genes and 8 housekeeping genes. The performance of each primer set for real time RT-PCR (qPCR) has been validated. Primer set information can be downloaded by clicking on the Resources tab and expanding the Technical Notes drop-down menu.

The PrimerArray Analysis Tool version 2.2*1 is capable of analyzing data obtained using PrimerArray Series products. This analysis tool is linked to the KEGG (Kyoto Encyclopedia of Genes and Genomes) database, allowing results to be visualized on a pathway map. Primer set information and the PrimerArray Analysis Tool can be downloaded by clicking on the Resources tab and expanding the Technical Notes drop-down menu. 

*1 The PrimerArray Analysis Tool version 2.2 uses a macro. Please select "enable macro" each time to use the file. 

Available PrimerArray Primer Sets

Human

  • PH001 - Cytokine-cytokine receptor interaction
  • PH002 - Cell cycle
  • PH003 - Cell adhesion molecules (CAMs)
  • PH004 - Jak-STAT signaling pathway
  • PH005 - Natural killer cell mediated cytotoxicity
  • PH006 - Axon Guidance
  • PH007 - Focal Adhesion 
  • PH008 - T Cell Receptor Signaling Pathway 
  • PH009 - TGF-beta Signaling Pathway
  • PH010 - Wnt Signaling Pathway 

Mouse

  • PN001 - Cytokine-cytokine receptor interaction
  • PN002 - Cell cycle
  • PN003 - Cell adhesion molecules
  • PN004 - Jak-STAT signaling pathway
  • PN005 - Natural killer cell mediated cytotoxicity
  • PN006 - Axon Guidance 
  • PN007 - Focal Adhesion 
  • PN008 - T Cell Receptor Signaling Pathway 
  • PN009 - TGF-beta Signaling Pathway 
  • PN010 - Wnt Signaling Pathway 

At-A-Glance Documents Images & Data Resources

Features

  • Affordability: Each primer set for real time RT-PCR (qPCR) supplies sufficient primer for up to 12 qPCR experiments (using a 25 µL qPCR reaction volume)
  • Minimizes pipetting steps and reduces error
  • Enables high-throughput gene expression analysis of biological pathways
  • Optimized for superior performance with SYBR Ex Taq qPCR kits
  • PrimerArray Analysis Tool makes data analysis easy and allows data visualization on pathway maps
  • Compatible with all standard real-time PCR instruments

Application

  • Gene expression analysis of biological pathways including cytokine-cytokine receptor interactions, cell cycling, cell adhesion, Jak-STAT signaling, natural killer cell mediated cytotoxicity, axon guidance, focal adhesion, T cell receptor signaling, TGF-beta signaling, Wnt signaling.

Procedural Overview for Gene Expression Analysis by Real Time RT-PCR

The overall workflow for the PrimerArray Series primer sets is straightforward:

  1. RNA extraction (30 min): NucleoSpin RNA II (Clontech Cat. #740955.50) or RNAiso (Cat.# 9108, not available in all geographic locations) is recommended.
  2. Reverse transcription (20 min): PrimeScript RT Master Mix (Perfect Real Time) (Cat. #RR036A) is recommended.
  3. Dispense real-time PCR reaction solution (10 min): Prepare a master mix containing the cDNA sample and SYBR Premix Ex Taq II (Tli RNase H Plus) (Cat.# RR820A); dispense into 96-well plate.
  4. Add PrimerArray primers (5 min): Use 4 µL PrimerArray Series primers per reaction
  5. Real-time PCR (1 - 2 hours): PrimerArray Series priemrs are compatible with all commonly used real-time PCR systems
  6. Data analysis (30 minutes): Use the PrimerArray Analysis Tool 

PrimerArray Analysis Tool

The PrimerArray analysis tool and user manual can be downloaded by clicking on the Resources tab and expanding the Technical Notes drop-down menu. 

Primer Sets for Real Time RT-PCR (qPCR): Information

To download information for each PrimerArray Series primer set, click on the Resources tab and expand the Technical Notes drop-down menu.

References

1. Hirano, S. et al. (2010) Toxicol. Applied Pharmacol. 249:8–15.

2. Yoshioka, R. et al. (2010) Invest. Ophthalmol. Vis. Sci. 51:5630-5639.




 
 
Products
Cat. # Product Package Size Price # of Units Select
PH006 PrimerArray® Axon guidance (Human) 12 x 96 Rxns $529.00
PN006 PrimerArray® Axon guidance (Mouse) 12 x 96 Rxns $529.00
PH003 PrimerArray® Cell Adhesion Molecules (Human) 12 x 96 Rxns $529.00
PN003 PrimerArray® Cell Adhesion Molecules (Mouse) 12 x 96 Rxns $529.00
PH002 PrimerArray® Cell Cycle (Human) 12 x 96 Rxns $529.00
PN002 PrimerArray® Cell Cycle (Mouse) 12 x 96 Rxns $529.00
PH001 PrimerArray® Cytokine-Cytokine Receptor Interaction (Human) 12 x 96 Rxns $529.00
PN001 PrimerArray® Cytokine-Cytokine Receptor Interaction (Mouse) 12 x 96 Rxns $529.00
PH007 PrimerArray® Focal adhesion (Human) 12 x 96 Rxns $529.00
PN007 PrimerArray® Focal adhesion (Mouse) 12 x 96 Rxns $529.00
PH004 PrimerArray® Jak-STAT Signaling Pathway (Human) 12 x 96 Rxns $529.00
PN004 PrimerArray® Jak-STAT Signaling Pathway (Mouse) 12 x 96 Rxns $529.00
PH005 PrimerArray® Natural Killer Cell Mediated Cytotoxicity (Human) 12 x 96 Rxns $529.00
PN005 PrimerArray® Natural Killer Cell Mediated Cytotoxicity (Mouse) 12 x 96 Rxns $529.00
PH008 PrimerArray® T Cell Receptor Signaling Pathway (Human) 12 x 96 Rxns $529.00
PN008 PrimerArray® T Cell Receptor Signaling Pathway (Mouse) 12 x 96 Rxns $529.00
PH009 PrimerArray® TGF-beta Signaling Pathway (Human) 12 x 96 Rxns $529.00
PN009 PrimerArray® TGF-beta Signaling Pathway (Mouse) 12 x 96 Rxns $529.00
PH010 PrimerArray® Wnt Signaling Pathway (Human) 12 x 96 Rxns $529.00
PN010 PrimerArray® Wnt Signaling Pathway (Mouse) 12 x 96 Rxns $529.00
 

 



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