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Products >  Real_Time_qPCR_Kits >  Primer_Set >  PrimerArray_Embryonic_Stem_Cell

RT-PCR Primers: PrimerArray Series for Embryonic Stem Cell and iPS Cell Screening

Gene expression analysis to screen for pluripotency of  embryonic stem cell and induced pluripotent stem cell (iPS cell) is streamlined and efficient with the PrimerArray Embryonic Stem Cell array. This array includes primers for key markers of mouse or human embryonic stem cell pluripotency as determined by the International Stem Cell Initiative International (ISCI). When performing gene expression analysis by real-time RT-PCR, these ready-to use primer sets save time, minimize pipetting, and reduce error.

The PrimerArray Embryonic Stem Cell array contains 96 primer pairs representing 88 genes associated with embryonic stem cell or iPS pluripotency and 8 housekeeping genes. When comparing an unknown sample to a control sample, gene expression differences can be expressed using the relative quantification method. The expression levels of multiple genes can be screened simultaneously. The PrimerArray Analysis Tool Ver. 2.2, available for download on this web page, and/or Multiplate RQ (for use with the Thermal Cycler Dice Real Time Systems, not available in all geographic locations) are useful for analyzing data obtained using these products. Primer set information and the PrimerArray analysis tool can be downloaded from this page (click on the Resources tab, then expand the drop-down menu under Technical Notes).

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Easily investigate the pluripotency of ES/iPS cells by real-time PCR: Measure key markers of human or mouse embryonic stem cells as determined by the International Stem Cell Initiative International (ISCI)
  • Affordable: Each PrimerArray Set supplies sufficient qPCR primers for up to 12 real-time PCR runs (25 µL reaction volume)
  • Reduces error by minimizing pipetting
  • Enables high-throughput analysis of stem cell pluripotency
  • Optimized for superior performance with SYBR Ex Taq qPCR kits
  • Straightforward data analysis with the PrimerArray Analysis Tool
  • Compatible with all standard real-time PCR systems

Applications

  • Pluripotency screens on embryonic stem cell and iPS cell samples
  • Screening for expression of stem cell marker genes in response to conditions or agents affecting pluripotency

Procedural Overview for Stem Cell Gene Expression Analysis by Real Time RT-PCR

The overall workflow for the PrimerArray Sets is straightforward:

  1. RNA extraction (30 min): We recommend NucleoSpin RNA II kits (Clontech Cat.#740955.50) or RNAiso (Cat.# 9108, not available in all geographic locations.)
  2. Reverse transcription (20 min): We recommend PrimeScript RT Master Mix (Perfect Real Time) (Cat.# RR036A)
  3. Dispense real-time PCR reaction solution (10 min): Prepare a master mix of your cDNA sample and SYBR Premix Ex Taq II (Tli RNase Plus) (Cat.# RR820A) and dispense into a 96-well plate.
  4. Add PrimerArray primers (5 min): Use 4 µL per reaction
  5. Real-time PCR (1 - 2 hours): Compatible with all commonly used real-time PCR systems
  6. Data analysis (30 minutes): PrimerArray Analysis Tool makes data analysis easy

PrimerArray Analysis Tool

The PrimerArray analysis tool facilitates data analysis and can be downloaded from this page (click on the Resources tab, then expand the drop-down menu under Technical Notes). For the PrimerArray Analysis Tool v2.2 User Manual, click on the Documents tab, then expand the drop-down menu under User Manuals.

 

Primer Information for RT-PCR Primer Sets

To download primer information for each PrimerArray RT-PCR primer set, click on the Resources tab, then expand the drop-down menu under Technical Notes.




 
 
Products
Cat. # Product Contents Size Price Units Select
PH016 PrimerArray® Embryonic Stem Cells (Human) 1 Set $519.00
PN016 PrimerArray® Embryonic Stem Cells (Mouse) 1 Set $519.00
 

 

Recommended Products
Cat. # Product Contents Size Price License Units Select
RR820A SYBR® Premix Ex Taq™ II (Tli RNase H Plus) 200 Rxns $290.00 License Statements
RR820B SYBR® Premix Ex Taq™ II (Tli RNase H Plus) 400 Rxns $566.00 License Statements
RR036A PrimeScript™ RT Master Mix (Perfect Real Time) 200 Rxns $425.00  
RR036B PrimeScript™ RT Master Mix (Perfect Real Time) 800 Rxns $1,365.00  
 

 

 
 

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