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Buffers for Protein Analyses

Takara Bio offers buffers and reagents for preparing cell lysates, protein electrophoresis (SDS-PAGE and Native-PAGE), and protein analysis. All buffers are premeasured in powder or tablet form and can be stored at room temperature. To prepare, simply dissolve the powder or tablet in the specified volume of distilled or deionized water.

Reagents for cell lysis

Product

Form

Use

IBLysis-I Buffer Solution (50 mL) Ready-to-use.
Tris-HCl buffer containing NP-40 and EDTA.
Sodium Dodecyl Sulfate (SDS) 10% Powder Powder containing pouches (2) Dissolve the contents of one pouch in distilled water to produce 1 L of 10% SDS.
Sodium Dodecyl Sulfate (SDS) 20% Powder Powder containing pouches (2) Dissolve the contents of one pouch in distilled water to produce 1 L of 20% SDS.

 

Protein electrophoresis (SDS-PAGE, Native-PAGE) and protein solubilization buffers

Product

Form

Use

Tris-Glycine Buffer (TG) Powder, pH8.3 Powder containing pouches (10) Dissolve the contents of one pouch in distilled water to produce 1 L of TG Buffer.
Tris-Glycine-SDS Buffer (TG-SDS) Powder, pH8.3 Powder containing pouches (10) Dissolve the contents of one pouch in distilled water to produce 1 L of TG-SDS Buffer.
Sodium Dodecyl Sulfate (SDS) 10% Powder Powder containing pouches (2) Dissolve the content of one pouch in distilled water to produce 1 L of 10% SDS.
Sodium Dodecyl Sulfate (SDS) 20% Powder Powder containing pouches (2) Dissolve the content of one pouch in distilled water to produce 1 L of 20% SDS.

 

ELISA and Western blot buffers

Product

Form

Use

Phosphate Buffered Saline (PBS) Tablets (100) Dissolve one tablet in distilled water to produce 1 L of PBS.
Phosphate Buffered Saline with Tween® 20 (PBS-T) Tablets (100) Dissolve one tablet in distilled water to produce 1 L of PBS-T
(Final solution contains 0.05% Tween 20.)
Tris Buffered Saline (TBS), pH7.6 Tablets (100) Dissolve one tablet in distilled water to produce 500 mL of TBS.
Tris Buffered Saline (TBS) Powder containing packages (30) Dissolve the content of one package in distilled water to produce 1 L of TBS.
Tris Buffered Saline with Tween20 (TBS-T), pH7.6 Tablets (100) Dissolve one tablet in distilled water to produce 500 mL of TBS-T
(Final solution contains 0.05% Tween 20.)
Western BLoT Blocking Buffer (Fish Gelatin) Solution (1 L) Ready-to-use.
A Western blot blocking buffer that contains gelatin extracted from fish in TBS-T buffer. It provides lower background because it is less likely to cross-react with mammalian antibodies than milk- and BSA-based blocking reagents.
Western BLoT Blocking Buffer (Protein Free) Solution (500 mL) Ready-to-use.
A Western blot blocking buffer that is completely chemically defined and free of protein components. It provides higher sensitivity in detecting normal proteins and is excellent for detecting phosphorylated proteins.
Western BLoT Stripping Buffer Solution (500 mL) Ready-to-use.
A reagent for removing primary and secondary antibodies from Western blot membranes so they can be reprobed with either a different concentration of primary antibody or an entirely different primary antibody.
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