Takara Bio offers buffers and reagents for preparing cell lysates, protein electrophoresis (SDS-PAGE and Native-PAGE), and protein analysis. All buffers are premeasured in powder or tablet form and can be stored at room temperature. To prepare, simply dissolve the powder or tablet in the specified volume of distilled or deionized water.
A Western blot blocking buffer that contains gelatin extracted from fish in TBS-T buffer. It provides lower background because it is less likely to cross-react with mammalian antibodies than milk- and BSA-based blocking reagents.
A Western blot blocking buffer that is completely chemically defined and free of protein components. It provides higher sensitivity in detecting normal proteins and is excellent for detecting phosphorylated proteins.
A reagent for removing primary and secondary antibodies from Western blot membranes so they can be reprobed with either a different concentration of primary antibody or an entirely different primary antibody.