Takara
login | register | order now | view cart | feedback
Products
Protein Research


Products >  Protein_Research >  Resources >  Protein_Buffer_Overview

Untitled Document

Buffers for Protein Analyses

Takara Bio offers buffers and reagents for preparing cell lysates, protein electrophoresis (SDS-PAGE and Native-PAGE), and protein analysis. All buffers are premeasured in powder or tablet form and can be stored at room temperature. To prepare, simply dissolve the powder or tablet in the specified volume of distilled or deionized water.

Reagents for cell lysis

Product

Form

Use

IBLysis-I Buffer Solution (50 mL) Ready-to-use
(Tris-HCl buffer containing NP-40 and EDTA)
Sodium Dodecyl Sulfate (SDS) 10% Powder Powder containing pouches (2) Dissolve the contents of one pouch in distilled water to produce 1 L of 10% SDS
Sodium Dodecyl Sulfate (SDS) 20% Powder Powder containing pouches (2) Dissolve the contents of one pouch in distilled water to produce 1 L of 20% SDS

 

Protein electrophoresis (SDS-PAGE, Native-PAGE) and protein solubilization buffers

Product

Form

Use

Tris-Glycine Buffer (TG) Powder, pH8.3 Powder containing pouches (10) Dissolve the contents of one pouch in distilled water to produce 1 L of TG Buffer
Tris-Glycine-SDS Buffer (TG-SDS) Powder, pH8.3 Powder containing pouches (10) Dissolve the contents of one pouch in distilled water to produce 1 L of TG-SDS Buffer
Sodium Dodecyl Sulfate (SDS) 10% Powder Powder containing pouches (2) Dissolve the content of one pouch in distilled water to produce 1 L of 10% SDS
Sodium Dodecyl Sulfate (SDS) 20% Powder Powder containing pouches (2) Dissolve the content of one pouch in distilled water to produce 1 L of 20% SDS

 

ELISA and Western blot buffers

Product

Form

Use

Phosphate Buffered Saline (PBS) Tablets (100) Dissolve one tablet in distilled water to produce 1 L of PBS
Phosphate Buffered Saline with Tween® 20 (PBS-T) Tablets (100)

Dissolve one tablet in distilled water to produce 1 L of PBS-T
(Final solution contains 0.05% Tween® 20)

Tris Buffered Saline (TBS), pH7.6 Tablets (100) Dissolve one tablet in distilled water to produce 500 mL of TBS
Tris Buffered Saline (TBS) Powder containing packages (30) Dissolve the content of one package in distilled water to produce 1 L of TBS
Tris Buffered Saline with Tween20 (TBS-T), pH7.6 Tablets (100) Dissolve one tablet in distilled water to produce 500 mL of TBS-T
(Final solution contains 0.05% Tween® 20)

 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions