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Protease Inhibition
Protein Folding and Expression
Protein Sequencing and Analysis
    N-Terminal Deblocking and Analysis
    Protein Fragmentation
         Arginylendopeptidase
         Endoproteinase Asp-N
         Pfu Protease S


Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  Protein_Fragmentation >  Pfu_Protease_S

Pfu Protease S

Pfu Protease S is an endo-type serine protease with broad specificity for native and denatured proteins. Cleavage occurs mainly on the carboxy side of peptide bonds of hydrophobic amino acid residues.

At-A-Glance Documents

Applications

  • Fragmentation of proteins and peptides required for primary structure analysis

Source

Bacillus species carrying a plasmid that encodes the Pyrococcus furiosus protease gene

Purity

Homogeneous on SDS-PAGE. No other proteases detected.

Properties

Molecular weight:45 kDa (SDS-PAGE)
42.906 kDa (calculated)
Optimum temperature:85–95°C
Optimum pH: 6.0–8.0
Inhibitor:PMSF
Tolerance to denaturants at 95°C pH 7.0:
 1% SDS; retains 50% activity after 24 hrs. at 95°C, pH 7.0
 6.4 M urea; retains 70% activity after 1 hr. at 95°C, pH 7.0
 50% acetonitrile; retains 90% activity after 1 hr. at 95°C, pH 7.0

Definition of Activity

One unit of enzyme activity corresponds to the amount required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C, pH 7.0.

Form

Solution in 25 mM Tris-HCl (pH 7.6) containing 40% ethanol


 
 
Products
Cat. # Product Package Size Price License # of Units Select
7339 Pfu Protease S 500 Units $110.00 License Statements
 

 


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