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Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  Protein_Fragmentation >  Pfu_Protease_S

Protein Digestion for Structural Analysis: Pfu Protease S

Pfu Protease S is an endo-type serine protease with broad specificity for native and denatured proteins. Cleavage occurs mainly on the carboxy terminus of peptide bonds on hydrophobic amino acid residues.

  At-A-Glance   Documents   Images & Data

Applications

  • Digestion of proteins and peptides for primary structure analysis

Source

Recombinant Bacillus encoding the Pyrococcus furiosus protease gene

Purity

Homogeneous via SDS-PAGE. No other proteases detected.

Properties

Molecular weight:45 kDa (SDS-PAGE)
42.906 kDa (calculated)
Optimum temperature:85–95°C
Optimum pH: 6.0–8.0
Inhibitor:PMSF
Tolerance to denaturants at 95°C pH 7.0:
 1% SDS; retains 50% activity after 24 hrs. at 95°C, pH 7.0
 6.4 M urea; retains 70% activity after 1 hr. at 95°C, pH 7.0
 50% acetonitrile; retains 90% activity after 1 hr. at 95°C, pH 7.0

Definition of Activity

One unit of activity corresponds to the amount of enzyme required to hydrolyze 1 mmol of N-succinyl Ala-Ala-Pro-Phe p-nitroanilide in 1 minute at 95°C and pH 7.0.

Form

In a solution of 25 mM Tris-HCl (pH 7.6) containing 40% ethanol




 
 
Products
Cat. # Product Contents Size Price License Units Select
7339 Pfu Protease S 500 Units $112.00 License Statements
 

 

 
 

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