- Fragmentation of proteins and peptides required for primary structure analysis
Pseudomonas fragi mutant
Homogeneous on SDS-PAGE. No other proteases detected.
- Molecular weight: 27 kDa (SDS-PAGE)
- Optimum temperature: 37°C
- Optimum pH: 6.0–8.5
- Inhibitors: 2-phenanthroline, EDTA, DTT
Definition of Activity
One unit of enzyme activity corresponds to the amount required to increase 0.001 absorbance unit of the peptide soluble in trichloro-acetic acid at 280 nm in 1 minute at 37°C, pH 8.0 using casein as the substrate.
Activity: Approximately 14 U/µg protein
Lyophilized (containing the equivalent of 50 µL of 10 mM Tris-HCl, pH 7.5)