Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Protein Research

Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  Protein_Fragmentation >  Endoproteinase_Asp-N

Protein Fragmentation for Sequencing: Endoproteinase Asp-N

Endoproteinase Asp-N is a metalloprotease that hydrolyzes peptide bonds on the amino terminus of aspartic and cysteic acid (oxidized cysteine). If the cysteine residue is reduced or alkylated, Endoproteinase Asp-N will cleave only the amino terminus of aspartic acid residues. The enzyme is supplied with a 250 mM sodium phosphate buffer (pH 8.0).

  At-A-Glance   Documents   Images & Data   Resources


  • Fragmentation of proteins and peptides required for primary structure analysis


Pseudomonas fragi mutant


Homogeneous on SDS-PAGE. No other proteases detected.


  • Molecular weight: 27 kDa (SDS-PAGE)
  • Optimum temperature: 37°C
  • Optimum pH: 6.0–8.5
  • Inhibitors: 2-phenanthroline, EDTA, DTT

Definition of Activity

One unit of enzyme activity corresponds to the amount required to increase 0.001 absorbance unit of the peptide soluble in trichloro-acetic acid at 280 nm in 1 minute at 37°C, pH 8.0 using casein as the substrate.

Activity: Approximately 14 U/µg protein


Lyophilized (containing the equivalent of 50 µL of 10 mM Tris-HCl, pH 7.5)

Cat. # Product Contents Size Price Units Select
7329 Endoproteinase Asp-N 2 ug $147.00



Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions