- Peptide bond cleavage
- Protein/peptide fragmentation prior to structural analysis
|Component:||250 mM Sodium acetate (pH 5.0), 50 mM DT, 5 mM EDTA|
Jackbean (Canavalia ensiformis)
No other proteases detected.
Protein samples must be denatured by chemical methods such as carboxymethylation prior to digestion with this enzyme.
|Molecular weight:||37.000 kDa (SDS-PAGE)|
|Stable pH range:||4.5–6.5|
|Thermal stability:||Stable below 50°C|
|Inhibitors:||p-chloromercuribenzoate (PCMB); N-ethylmaleimide (NEM)|
|Tolerance to denaturants:|
| Less than or equal to 2 M urea|
| ||Less than or equal to 0.5 M guanidine-HCl|
| ||Less than or equal to 0.5% SDS|
In a solution of 20 mM sodium acetate buffer (pH 5.0) containing 50% glycerol, 0.005% Brij-35, 1 mM DTT and 1 mM EDTA
0.2 mU/vial (5–10 uses for digestion of 2 nmol protein)
Definition of Activity
One unit of activity corresponds to the amount of enzyme required to produce 1 µmol of DNP-Pro-Glu-Ala from DNP-Pro-Glu-Ala-Asn-NH2 in 1 minute at 37°C (pH 5.0).