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Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  Protein_Fragmentation >  Asparaginylendopeptidase

Protein Analysis: Asparaginylendopeptidase

Asparaginylendopeptidase specifically cleaves peptide bonds on the carboxyl side of asparagine residues including –Asn-Pro– bonds. The peptide bond cleavage specificity of this enzyme has been validated on many peptides and proteins, with bonds located on N-terminal or N-second position asparagine residues not cleaved with this enzyme. Only those bonds adjacent to C-terminal asparagines residues are cleaved. Because asparagine residues bound to sugar chains are not cleaved, this enzyme can be used for protein analysis of sequenced glycoproteins. Asparaginylendopeptidase is isolated from Jackbean (Canavalia ensiformis).

At-A-Glance Documents Images & Data Resources

Applications

  • Peptide bond cleavage
  • Protein/peptide fragmentation prior to structural analysis

Components

5X Buffer

Volume:1 mL
Component:250 mM Sodium acetate (pH 5.0), 50 mM DT, 5 mM EDTA

Source

Jackbean (Canavalia ensiformis)

Purity

No other proteases detected.

Storage

–20°C

Notes

Protein samples must be denatured by chemical methods such as carboxymethylation prior to digestion with this enzyme.

Properties

Molecular weight:37.000 kDa (SDS-PAGE)
Optimum pH:5.5–6.5
Stable pH range:4.5–6.5
Optimum temperature:37–45°C
Thermal stability:Stable below 50°C
Inhibitors:p-chloromercuribenzoate (PCMB); N-ethylmaleimide (NEM)
Tolerance to denaturants:
 Less than or equal to 2 M urea
 Less than or equal to 0.5 M guanidine-HCl
 Less than or equal to 0.5% SDS

Form

In a solution of 20 mM sodium acetate buffer (pH 5.0) containing 50% glycerol, 0.005% Brij-35, 1 mM DTT and 1 mM EDTA

Volume

0.2 mU/vial (5–10 uses for digestion of 2 nmol protein)

Definition of Activity

One unit of activity corresponds to the amount of enzyme required to produce 1 µmol of DNP-Pro-Glu-Ala from DNP-Pro-Glu-Ala-Asn-NH2 in 1 minute at 37°C (pH 5.0).




 
 
Products
Cat. # Product Package Size Price # of Units Select
7319 Asparaginylendopeptidase 0.2 mU $367.00
 

 


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