Applications - Fragmentation of proteins and peptides prior to structural analysis
ComponentsSupplied Buffer (5X)| Volume: | 1 mL | | Component: | 250 mM Sodium acetate, pH 5.0, 50 mM DT, 5 mM EDTA |
SourceJack bean
PurityNo other proteases detected.
Storage–20°C
NotesIt is necessary to denature the protein samples by chemical procedure such as carboxymethylation, prior to digestion with this enzyme.
Properties | Molecular weight: | 37,000 (SDS-PAGE) | | Optimum pH: | 5.5–6.5 | | Stable pH range: | 4.5–6.5 | | Optimum temperature: | 37–45°C | | Thermal stability: | Stable below 50°C | | Inhibitors: | p-chloromercuribenzoate (PCMB); N-ethylmaleimide (NEM) | | Tolerance to denaturants: | | Stable against | Less than or equal to 2 M urea | | | Less than or equal to 0.5 M guanidine-HCl | | | Less than or equal to 0.5% SDS |
FormSolution in 20 mM sodium acetate buffer (pH 5.0) containing 50% glycerol, 0.005% Brij-35, 1 mM DTT and 1 mM EDTA
Volume0.2 mU/vial (5–10 uses for digestion of 2 nmol protein)
Definition of ActivityOne unit of enzyme activity corresponds to the amount required to produce 1 µmol of DNP-Pro-Glu-Ala from DNP-Pro-Glu-Ala-Asn-NH2 in 1 minutes at 37°C, pH 5.0.
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