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Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  N-Terminal_Deblocking_and_Analysis >  Pfu_Pyroglutamate_Aminopeptidase

Edman Degradation and Protein Sequencing: Pfu Pyroglutamate Aminopeptidase

Pfu Pyroglutamate Aminopeptidase removes pyroglutamic acids from the N-termini of proteins and peptides, enabling protein sequencing following Edman degradation.

This enzyme may work well with some intact and non-denatured proteins; a denaturation step may be unnecessary in all cases. Pfu Pyroglutamate Aminopeptidase is supplied with a 5X Reaction Buffer [250 mM sodium phosphate (pH 7.0), 50 mM DTT, 5 mM EDTA].

At-A-Glance Documents Images & Data

Applications

  • Removal of pyroglutamic acids from the N-termini of proteins and peptides

Components

Supplied Buffer

Volume: 1 mL
Component: 250 mM sodium phosphate buffer (pH 7.0) containing 50 mM DTT and 5 mM EDTA
Comparison of specific activities across different temperatures: 5.83 U/mg protein at 37°C showed activities of 12.2 U/mg protein or 28.3 U/mg protein at 50°C or 75°C, respectively.

Source

Recombinant Escherichia coli encoding the Pyrococcus furiosus pyroglutamate aminopeptidase gene.

Purity

Greater than or equal to 90% via SDS-PAGE. No other protease activity detected.

Properties

Systematic Name:L-Pyrrolidone carboxyl peptidase
Enzyme code:3.4.19.3
Molecular weight:24.072 kDa (calculated)
28 kDa (by SDS-PAGE)
Optimum temperature: 95–100°C
Thermal stability:~90% activity at 75°C
pH 7.0, 150 min.
Optimum pH:6.0–9.0
Stable pH range:5.0–9.0
Greater than or equal to 80% activity in a pH range of 5.0-9.0 at 75°C when reactived for 30 min.
Tolerance to denaturants:Less than or equal to 1 M Urea
Less than or equal to 1 M Guanidine-HCl
Less than or equal to 0.01% SDS
Inhibitors:PCMB, Hg2+

Definition of Activity

One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol pyroglutamate p-nitroanilide at 37°C in 1 minute at pH 7.0.

References

  1. Shimada, Y. et al. (1989) J. Biochem. 106:383-388.
  2. Hamazume, Y. et al. (1987) J. Biochem. 101:217-223.



 
 
Products
Cat. # Product Package Size Price # of Units Select
7334 Pfu Pyroglutamate Aminopeptidase 10 mU $248.00
 

 


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