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Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  N-Terminal_Deblocking_and_Analysis >  Pfu_Pyroglutamate_Aminopeptidase

Pfu Pyroglutamate Aminopeptidase

Pfu Pyroglutamate Aminopeptidase removes Pyroglutamic Acids from the N-termini of proteins and peptides and deblocks N-terminal pyroglutamates of proteins and peptides for sequence analysis using Edman Degradation.

This enzyme may work well with some intact, non-denatured proteins and, thus, the denaturation step may be unnecessary in these instances. Pfu Pyroglutamate Aminopeptidase is supplied with 5X Reaction Buffer [250 mM sodium phosphate (pH 7.0), 50 mM DTT, 5mM EDTA].

At-A-Glance Documents Images & Data

Applications

  • Removal of pyroglutamic acids from the N-termini of proteins and peptides
  • Deblocking of N-terminal pyroglutamates of proteins and peptides for sequence analysis using edman degradation

Components

Supplied Buffer

Volume: 1 mL
Component: 250 mM sodium phosphate buffer (pH 7.0) containing 50 mM DTT and 5 mM EDTA
Comparison of specific activities at various temperatures (an example): Enzyme with a specific activity of 5.83 U/mg protein at 37°C showed the activities of 12.2 U/mg protein or 28.3 U/mg protein at 50°C or 75°C, respectively.

Source

Escherichia coli carrying plasmids encoding the Pyrococcus furiosus pyroglutamate aminopeptidase gene.

Purity

Greater than or equal to 90% on SDS-PAGE. No other proteases detected.

Properties

Systematic Name:L-Pyrrolidone carboxyl peptidase
Enzyme code:3.4.19.3
Molecular weight:24.072 kDa (calculated)
28 kDa (by SDS-PAGE)
Optimum temperature: 95–100°C
Thermal stability:approx. 90% activity at 75°C
+ pH 7.0, 150 min.
Optimum pH:6.0–9.0
Stable pH range:5.0–9.0
Greater than or equal to 80% activity in a pH range of 5.0-9.0 at 75°C when reactived for 30 min.
Tolerance to denaturants:Less than or equal to 1 M Urea
Less than or equal to M Guanidine-HCl
Less than or equal to 0.01% SDS
Inhibitors:PCMB, Hg2+

Definition of Activity

One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol pyroglutamate p-nitroanilide at 37°C in 1 minute, pH 7.0.

References

  1. Shimada, Y. et al. (1989) J. Biochem. 106:383.
  2. Hamazume, Y. et al. (1987) J. Biochem, 101:217.



 
 
Products
Cat. # Product Package Size Price # of Units Select
7334 Pfu Pyroglutamate Aminopeptidase 10 mU $243.00
 

 


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