Applications - Liberates the N-terminal acyl blocking group from proteins
Components Supplied Buffer (5X)| Volume: | 1 mL | | Component: | 250 mM N-Ethylmorpholine-AcOH buffer | | | (pH 8.0) containing 0.5 mM CoCl2 | | Once thawed, the buffer should be stored at 4°C. Avoid freeze-thaw cycles. |
SourceYeast carrying the plasmid which contains the gene encoding Pyrococcus furiosus DAP.
PurityHomogeneous on PAGE
Storage–20°C. Once thawed, the enzyme solution should be stored at 4°C. The thawed enzyme and buffer are stable at least 3 months at 4°C.
NotesSince this enzyme cannot react with proteins containing the higher order structures, protein samples should be denatured by chemical pocedures such as carboxymethylation. This enzyme cannot act on proteins blotted on PVDF membrane.
Properties - Specific Activity: 8–10 units/mg
- Protein Concentration: 1 mg/mL
- Molecular weight: 38,639 kDa (mass spectrometry) 451,000 kDa (sedimentation equilibrium method)
- Activator: CoCl2
- Inhibitor: Amastatin, EDTA
- Optimum pH: 6.5–9.0
- Optimum Temperature: 85–90°C
- Thermal Stability: It was confirmed that the enzyme retains 100% activity after 48 hrs. at 50°C in the supplied buffer (pH 8.0, with 0.1 mM Co2+).
FormSolution in 50 mM N-Ethylmorpholine-AcOH buffer (pH 8.0)
Definition of ActivityOne unit of the enzyme activity corresponds to the amount required to hydrolyze 1 µmol of Leucine-p-nitroanilide at 75°C, pH 8.0, in one minute.
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