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Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  N-Terminal_Deblocking_and_Analysis >  Pfu_Methionine_Aminopeptidase

Pfu Methionine Aminopeptidase

Pfu Methionine Aminopeptidase is a thermostable Methionine Aminopeptidase isolated from Pyrococcus furiosus and produced as a recombinant protein. It specifically liberates the N-terminal methionine residue from proteins and peptides.

At-A-Glance Documents

Applications

  • The Release of Met residue from recombinant proteins and peptides produced by recombinant technology

Source

Escherichia coli carrying plasmids encoding the Pyrococcus furiosus methionine aminopeptidase gene.

Purity

Greater than or equal to 95% on SDS-PAGE. No other proteases detected.

Storage

–20°C

Properties

Molecular weight:32.848 (mass spectrom analysis)
37,000 (SDS-PAGE)
Optimum temperature:85–95°C
Thermostability:This enzyme is stable for 1 hour at 75°C (pH 7.2, 0.5 mM Co2+)
Optimum pH: 7.0–8.0
Tolerance to denaturants:Less than or equal to 2 M Urea
Less than or equal to 0.2 M Guanidine-HCl
Less than or equal to 0.01% SDS
Activator: Co2+
Inhibitor:EDTA

Form

Solution in 10 mM Tris-HCl, pH 7.5, containing 0.01% Tween 20 and 0.1 mM CoCl2

References

  1. Yasueda, H. et al. (1990) BioTechnology 8:1036.
  2. Ben-Bassat, A. et al. (1987) J. Bacteriol. 169:751.
  3. Miller, C.G. et al. (1987) Proc. Natl. Acad. Sci. USA 84:2718.
  4. Ben-Bassat, A. et al. (1987) Nature 326:315.



 
 
Products
Cat. # Product Package Size Price # of Units Select
7335 Pfu Methionine Aminopeptidase 20 mU $221.00
 

 


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