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Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  N-Terminal_Deblocking_and_Analysis >  Pfu_Methionine_Aminopeptidase

Edman Degradation and Protein Sequencing: Pfu Methionine Aminopeptidase

Pfu Methionine Aminopeptidase is a thermostable methionine aminopeptidase isolated from Pyrococcus furiosus and expressed as a recombinant protein. It specifically liberates the N-terminal methionine residue from proteins and peptides during Edman degradation, enabling protein sequencing.

  At-A-Glance   Documents   Images & Data

Applications

  • Release of methionine residues from recombinant proteins and peptides

Source

Escherichia coli transformed with Pyrococcus furiosus methionine aminopeptidase gene.

Purity

Greater than or equal to 95% via SDS-PAGE. No other proteases detected.

Storage

–20°C

Properties

Molecular weight:32.848 (MS analysis)
37,000 (SDS-PAGE)
Optimum temperature:85–95°C
Thermostability:This enzyme is stable for 1 hour at 75°C, pH 7.2 and 0.5 mM Co2+
Optimum pH: 7.0–8.0
Tolerance to denaturants:Less than or equal to 2 M Urea
Less than or equal to 0.2 M Guanidine-HCl
Less than or equal to 0.01% SDS
Activator: Co2+
Inhibitor:EDTA

Form

Solution of 10 mM Tris-HCl, pH 7.5, containing 0.01% Tween 20 and 0.1 mM CoCl2

References

  1. Yasueda, H. et al. (1990) Biotechnology 8:1036-1040.
  2. Ben-Bassat, A. et al. (1987) J. Bacteriol. 169:751-757.
  3. Miller, C.G. et al. (1987) Proc. Natl. Acad. Sci. USA 84:2718-2722.
  4. Ben-Bassat, A. et al. (1987) Nature 326:315.



 
 
Products
Cat. # Product Contents Size Price Units Select
7335 Pfu Methionine Aminopeptidase 20 mU $225.00
 

 

 
 

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