- Liberates the N-terminal amino acids up to X-Pro from proteins and peptides
Recombinant Escherichia coli encoding the Pyrococcus furiosus aminopeptidase I gene.
Homogeneous on SDS-PAGE
|Molecular weight:||37.483 kDa (calculated) 36-37 kDa (SDS-PAGE)|
|Inhibitor:||EDTA (Completely inhibited at 0.1 mM)|
|Optimum pH:||5.5–8.0 (in the presence of 20 µM Co2+ at 90°C)|
|Optimum Temperature:||80°C (in the presence of 20 µM Co2+ and pH 6.0) 95°C (without Co2+ at pH 6.0)|
|Thermal Stability: ||65% activity after 4 hrs. at 90°C (without Co2+ at pH 8.0).|
Definition of Activity
One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol of Leucine-p-nitroanilide at 75°C, pH8.0 in one minute.
- > 16 U/mg protein (without Co2+)
- > 80 U/mg protein (in the presence of 20 µM Co2+)