Applications - Liberates the N-terminal amino acids up to X-Pro from proteins and peptides
SourceEscherichia coli carrying plasmids encoding the Pyrococcus furiosus aminopeptidase I gene.
PurityHomogeneous on SDS-PAGE
Properties | Molecular weight: | 37.483 kDa (calculated) 36-37 kDa (SDS-PAGE) | | Isoelectric point: | 4.6–4.65 | | Inhibitor: | EDTA (Completely inhibited at 0.1 mM) | | Optimum pH: | 5.5–8.0 (in the presence of 20 µM Co2+, at 90°C) | | Optimum Temperature: | 80°C (in the presence of 20 µM Co2+, pH 6.0) 95°C (without Co2+, pH 6.0) | | Thermal Stability: | The enzyme retains 65% activity after 4 hrs. at 90°C (pH 8.0, without Co2+). |
Definition of ActivityOne unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol of Leucine-p-nitroanilide at 75°C, pH 8.0, in 1 minute. Activities- Approximately 84 U/mg protein (5 mM Leucine-p-nitroanilide)
- Approximately 344 U/mg protein (in the presence of 20 µM Co2+, 5 mM Leucine-p-nitroanilide)
FormLyophilized
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