Takara
login | register | order now | view cart | feedback
Products
Protein Research


Products >  Protein_Research >  Protein_Sequencing_and_Analysis >  N-Terminal_Deblocking_and_Analysis >  Pfu_Aminopeptidase_I

Edman Degradation and Protein Sequencing: Pfu Aminopeptidase I

Edman degradation is a commonly used method for protein sequencing via N-terminal amino acid cleavage. The Pfu Aminopeptidase I is a thermostable exo-type aminopeptidase isolated from Pyrococcus furiosus. It is expressed as a recombinant protein and liberates N-terminal amino acids from proteins and peptides. While Pfu Aminopeptidase I has a wide range of substrate specificities, it does not hydrolyze peptide bonds at the α-amino group of proline (X-Pro). Enzyme activity is significantly increased in the presence of Co2+.

At-A-Glance Documents Images & Data

Applications

  • Liberates the N-terminal amino acids up to X-Pro from proteins and peptides

Source

Recombinant Escherichia coli encoding the Pyrococcus furiosus aminopeptidase I gene.

Purity

Homogeneous on SDS-PAGE

Properties

Molecular weight:37.483 kDa (calculated) 36-37 kDa (SDS-PAGE)
Isoelectric point:4.6–4.65
Inhibitor:EDTA (Completely inhibited at 0.1 mM)
Optimum pH:5.5–8.0 (in the presence of 20 µM Co2+ at 90°C)
Optimum Temperature:80°C (in the presence of 20 µM Co2+ and pH 6.0) 95°C (without Co2+ at pH 6.0)
Thermal Stability: 65% activity after 4 hrs. at 90°C (without Co2+ at pH 8.0).

Definition of Activity

One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol of Leucine-p-nitroanilide at 75°C, pH8.0 in one minute.

Activities

  • > 16 U/mg protein (without Co2+)
  • > 80 U/mg protein (in the presence of 20 µM Co2+)

Form

Lyophilized




 
 
Products
Cat. # Product Package Size Price # of Units Select
7336 Pfu Aminopeptidase I 0.5 mg $135.00
 

 


Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions