Takara's pCold Expression Vectors offer cold shock expression technology for high purity and high yield recombinant protein expression.
The pCold DNA Cold Shock Expression System includes three different vectors, with each vector containing the cold shock protein A (cspA) promoter for expression of high purity and high yield recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) while the bacterial cell is in a state of suppressed host protein expression and decreased protease activity. This results in a higher yield of target protein (at up to 60% of the intracellular protein). In addition to the cspA promoter, all three vectors contain a lac operon (for expression control), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment and multiple cloning site (MCS). Two of the vectors also contain a translation enhancing element (TEE). pCold II contains a His tag sequence. These vectors work equally well for non-labeled and radiolabeled recombinant protein expression and can be used in conjunction with Takara's Chaperone Plasmid Set.