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Products >  Protein_Research >  Protein_Folding_and_Expression >  mRNA_Interferase-MazF_Enzyme

mRNA Interferase-MazF Enzyme

MazF Enzyme is a toxin protein in the toxin-antitoxin module of E. coli. It possesses endoribonuclease activity and specifically cleaves single-stranded RNA (ssRNA) at 5' ACA sequences. MazF does not cleave double-stranded RNA (dsRNA), double-stranded DNA or single-stranded DNA. The mRNA Interferase-MazF Enzyme is supplied as a fusion protein of E. coli MazF and Trigger Factor, which is an E. coli chaperone protein. The enzyme is also supplied with 5X MazF Buffer consisting of 200 mM sodium phosphate (pH 7.5) and 0.05% Tween-20.

At-A-Glance Documents Images & Data

Applications

  • Site-dependent cleavage of ssRNA

Source

Expressed as a recombinant protein in Escherichia coli.

Purity

Additional MazF nuclease activities were not detected under any of the following conditions as demonstrated by RNA or DNA electrophoresis:

  1. After incubation with 40 pmol non-AC sequence ssRNA and 20 units mRNA Interferase-MazF for 16 hours at 37°C and pH 7.5.
  2. After incubation with 40 pmol dsRNA and 20 units mRNA Interferase-MazF for 16 hours at 37°C and pH 7.5.
  3. After incubation with 1 mg lambda DNA-Hind III fragments and 20 units mRNA Interferase-MazF for 16 hours at 37°C and pH 7.5.

Notes

Licensing agreement required. This product is limited to research use only. Product must not be used for clinical or in vitro diagnostic purposes.

Form

Provided as a solution of 20 mM sodium phosphate (pH 6.0), 0.01% Tween-20 and 50% glycerol.

Definition of Activity

One unit is defined as the amount of enzyme that cleaves 1 pmol of standard substrate ROX-5'-GATAUACATATCT-eclipse (the underlined region is composed of RNA) after 10 min. at 37°C and pH 7.5. Buffer composition used for activity definition: 20 mM sodium phosphate, pH 7.5 and 0.05% Tween-20.

References

  1. Suzuki, M. et al. (2005) Mol. Cell 18:253-261.
  2. Zhang. Y. et al. (2004) J. Biol. Chem. 280:3143-3150.
  3. Zhang, Y. et al. (2003) Mol. Cell 12:913-923.



 
 
Products
Cat. # Product Package Size Price License # of Units Select
2415A ◊mRNA Interferase™-MazF 1,000 Units $643.00 License Statements
 

 



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