Source

Expressed as a recombinant protein in Escherichia coli.

Purity

Other nuclease activities were not detected under any of the following conditions as demonstrated by RNA or DNA electrophoresis patterns:

1. After incubation of 40 pmol of ssRNA lacking AC sequences with 20 units of mRNA Interferase-MazF for 16 hours at 37°C and pH 7.5.
2. After incubation with 40 pmol of dsRNA with 20 units of mRNA Interferase-MazF for 16 hours at 37°C and pH 7.5.
3. After incubation of 1 mg of lambda DNA-Hind III fragments with 20 units of mRNA Interferase-MazF for 16 hours at 37°C and pH 7.5.

Notes

Licensing agreement required. The use of this product is limited for research purposes. It must not be used for clinical or in vitro diagnosis purposes.

Form

Solution in 20 mM Sodium phosphate (pH 6.0), 0.01% Tween-20, 50% glycerol.

Definition of Activity

One unit is the amount of enzyme that cleaves 1 pmol of standard substrate (ROX-5'-GATAUACATATCT-eclipse, the underlined regions are composed of RNA) for 10 min. at 37°C and pH 7.5. Buffer composition use for activity definition: 20 mM Sodium phosphate, pH 7.5 and 0.05% Tween-20.

References

1. Suzuki, M. et al. (2005) Molecular Cell 18:253-261.
2. Zhang. Y. et al. (2004) Journal of Biological Chemistry 280:3143-3150.
3. Zhang, Y. et al. (2003) Molecular Cell 12:913-923.