The Refolding CA Kit uses a novel artificial chaperone technology in an easy 2-step procedure for optimizing the refolding conditions of inclusion body proteins. Optimization results in correct protein folding and restoration of protein activity.
The small kit is supplied with guanidine hydrochloride and DTT for protein unfolding, four different surfactants that can be added independently to the unfolded protein solution to provide protection against molecular aggregation, and highly polymerized cycloamylose (CA), an artificial chaperone, for surfactant removal and recovery of protein activity. Overnight incubation of the CA-treated protein is followed by a quick 10-minute centrifugation and collection of the supernatant containing the refolded protein.
The large kit is used for large scale refolding after the reaction conditions have been determined using the small Refolding CA kit and consists of only denaturant and CA.
The artificial chaperone technology in the Refolding CA Kit is licensed from NFRI, BTRAI, and Ezaki Glico Co, Ltd.
