Takara HRV 3C Protease is a highly purified recombinant protein derived from human Rhinovirus type 14 and expressed in E. coli. 3C Protease cleaves a specific amino acid sequence (LeuGluValLeuPheGln ↓ GlyPro) and can be used to cleave fusion tag sequences from recombinant proteins that contain an HRV 3C protease cleavage site.
This 3C protease also contains an N-terminal 6xHN tag (modified his-tag), and thus can be easily eliminated from the protease reaction solution through immobilized metal affinity chromatography (IMAC) using TALON® Metal Affinity Resin or His60 Ni Superflow™ Resin. In addition, because the enzyme is active at 4°C, protein cleavage reactions can be performed under conditions that do not affect activity or stability of the target protein.
This product also includes a Cleavage Control Fusion Protein for use as a positive control. The Control Protein has an N-terminal 6xHN tag, and digestion of the protein with HRV 3C Protease results in a 52 kDa TF peptide and a 24 kDa GST peptide that can be visualized by SDS-PAGE.