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Products >  Protein_Research >  Protein_Folding_and_Expression >  HRV_3C_Protease

HRV 3C Protease for Fusion Tag Cleavage from Recombinant Proteins

Takara HRV 3C Protease is a highly purified recombinant protein derived from human Rhinovirus type 14 and expressed in E. coli. 3C Protease cleaves a specific amino acid sequence (LeuGluValLeuPheGln ↓ GlyPro) and can be used to cleave fusion tag sequences from recombinant proteins that contain an HRV 3C protease cleavage site.

This 3C protease also contains an N-terminal 6xHN tag (modified his-tag), and thus can be easily eliminated from the protease reaction solution through immobilized metal affinity chromatography (IMAC) using TALON® Metal Affinity Resin or His60 Ni Superflow™ Resin. In addition, because the enzyme is active at 4°C, protein cleavage reactions can be performed under conditions that do not affect activity or stability of the target protein.

This product also includes a Cleavage Control Fusion Protein for use as a positive control. The Control Protein has an N-terminal 6xHN tag, and digestion of the protein with HRV 3C Protease results in a 52 kDa TF peptide and a 24 kDa GST peptide that can be visualized by SDS-PAGE.

At-A-Glance Documents

Features

  • Active at 4°C, allowing the cleavage reaction to proceed under conditions that do not affect the activity or stability of the recombinant target protein
  • 6xHN tag that allows removal of the 3C protease from the reaction using IMAC

Application

  • Cleaving fusion tags (e.g., His, TF, ProS2, GST) from recombinant fusion proteins
 

Components

HRV 3C Protease (1 U/µL) 500 Units
Cleavage Control Fusion Protein (1 µg/µL)
10 µL
10X HRV 3C Cleavage Buffer
10 mL

Activity Definition

1 unit (U) is defined as the amount of enzyme that cleaves at least 95% of the Cleavage Control Fusion Protein (100 µg in 1X HRV 3C Cleavage Buffer) in 16 hours at 4°C.

Storage

-20°C




 
 
Products
Cat. # Product Package Size Price License # of Units Select
7360 HRV 3C Protease 500 Units $220.00 License Statements
 

 

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