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Detection of Horseradish Peroxidase (HRP): Western BLoT Chemiluminescent Substrate Series

The Western BLoT HRP Substrate series are chemiluminescent substrates for detecting horseradish peroxidase (HRP)-labeled antibodies used during Western blotting. All of the substrates contain two-components, a peroxide reagent and a luminol/enhancer solution. To use, equal volumes of the two solutions are mixed, the blot is incubated with the solution, and chemiluminescence is detected using film or digital imaging equipment. Takara Bio offers four types of HRP substrates for Western blot detection; please refer to the table in the At-A-Glance section to compare the substrates' specifications.

  • The Western BLoT Chemiluminescence HRP Substrate (Cat. # T7101A) provides sensitivity suitable for detection using X-ray film. Although it is the most basic substrate in Western BLoT HRP Substrate series, it produces a strong signal and provides high sensitivity with short exposure times.
  • The Western BLoT Quant HRP Substrate (Cat. # T7102A) is intended for Western blot detection using a CCD camera. The signal from Western BLoT Quant is linear with respect to protein amount over a broad range of concentrations. In addition, this substrate produces a strong, long-lived signal, allowing long exposure times to enable the detection of low-abundance proteins. Western BLoT Quant can also be used for X-ray film detection.
  • The Western BLoT Hyper HRP Substrate (Cat. # T7103A) is more sensitive than Western BLoT Quant and is suitable for quantitative detection of low-abundance proteins. Western BLoT Hyper is also appropriate in situations when the amount of primary antibody is limited and a high dilution factor is desired, or when the primary antibody has a relatively low binding affinity. Western BLoT Hyper can also be used for X-ray film detection.
  • The Western BLoT Ultra Sensitive HRP Substrate (Cat. # T7104A) is the most sensitive substrate in the series and is suitable for quantitative detection of very low-abundance proteins. It is possible to detect protein levels in the femtogram range using this product.

At-A-Glance Documents Images & Data

Features

Product nameFeaturesDetection LimitDetection Method Membrane
Western BLoT Chemiluminescence HRP Substrate Stronger signal than other equivalent ECL substrates. Picogram X-ray film Nitrocellulose, PVDF
Western BLoT Quant HRP Substrate Long-lasting, stable signal. High sensitivity and quantitative accuracy. Low picogram CCD camera,
X-ray film
Nitrocellulose, PVDF
Western BLoT Hyper HRP Substrate Quantitative detection even for low-abundance proteins. Strong signal when high dilution factor for the primary antibody is used.Femtogram CCD camera,
X-ray film
Nitrocellulose, PVDF
Western BLoT Ultra Sensitive HRP Substrate Highest sensitivity in the series.Low femtogram CCD camera,
X-ray film
Nitrocellulose, PVDF

Application

  • Detection of Horseradish Peroxidase (HRP)-labeled antibodies for Western blotting

Components

Western BLoT Chemiluminescence HRP Substrate (Cat. # T7101A)

(100 mL, for 1000 cm2 of membrane)
Western BLoT Chemiluminescence Luminol/Enhancer Solution125 mL 
Western BLoT Chemiluminescence Peroxide Reagent125 mL 

Western BLoT Quant HRP Substrate (Cat. # T7102A)

(100 mL, for 1000 cm2 of membrane)
Western BLoT Quant Luminol/Enhancer Solution50 mL
Western BLoT Quant Peroxide Reagent50 mL

Western BLoT Hyper HRP Substrate (Cat. # T7103A)

(100 mL, for 1000 cm2 of membrane)
Western BLoT Hyper Luminol/Enhancer Solution50 mL
Western BLoT Hyper Peroxide Reagent50 mL

Western BLoT Ultra Sensitive HRP Substrate (Cat. # T7104A)

(100 mL, for 1000 cm2 of membrane)
Western BLoT Ultra Sensitive Luminol/Enhancer Solution50 mL
Western BLoT Ultra Sensitive Peroxide Reagent50 mL

Storage

4°C




 
 
Products
Cat. # Product Package Size Price License # of Units Select
T7101A Western BLoT Chemiluminescence HRP Substrate 250 mL $192.00  
T7101B Western BLoT Chemiluminescence HRP Substrate 500 mL $384.00  
T7102A Western BLoT Quant HRP Substrate 100 mL $245.00  
T7102B Western BLoT Quant HRP Substrate 200 mL $490.00  
T7103A Western BLoT Hyper HRP Substrate 100 mL $299.00  
T7103B Western BLoT Hyper HRP Substrate 200 mL $598.00  
T7104A Western BLoT Ultra Sensitive HRP Substrate 100 mL $495.00 License Statements
T7104B Western BLoT Ultra Sensitive HRP Substrate 200 mL $990.00 License Statements
 

 



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