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Products >  PCR_Products >  Real_Time_qPCR_Kits >  SYBR_Detection >  SYBR_Premix_Ex_Taq_Tli_RNaseH_Plus

SYBR Premix Ex Taq (Tli RNaseH Plus)

SYBR Premix Ex Taq (Tli RNaseH Plus) is a qPCR reagent specifically designed for intercalator-based real time PCR using SYBR Green I. It is supplied at a 2X concentration premixed with SYBR Green I at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures.

A combination of TaKaRa Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, and a buffer optimized for qPCR allows high amplification efficiency and high detection sensitivity in qPCR. The 2X premixed reagent also contains Tli RNaseH, a heat-resistant RNase H, which minimizes inhibition of the qPCR reaction due to residual mRNA when using cDNA as template. This product is suitable for high-speed qPCR and allows accurate assay and detection of targets, making it possible to conduct real time PCR analyses with excellent reproducibility and reliability.

Two ROX Reference Dyes are also supplied as separate components with Cat.# RR420A, RR420B, RR420L and RR420W. These serve as convenient internal reference standards for use in normalizing signals due to non-PCR related fluorescence fluctuations that occur either between wells or over time in different instruments.

Cat.# RR420L and RR420W are larger package sizes (5 mL and 25 mL (5 x 5 mL), respectively) of SYBR Premix Ex Taq (Tli RNase H Plus). Each of these provides convenient larger packaging, and is supplied with a single, separate vial of ROX Reference Dye.

Cat.# RR42LR and RR42WR are larger package sizes (5 mL and 25 mL (5 x 5 mL), respectively), that contain ROX Reference Dye in the premix.
At-A-Glance Documents Resources

Features

  • Minimizes qPCR inhibition due to residual mRNA when using cDNA as a template
  • Hot start PCR enzyme that allows high efficiency amplification and high sensitivity detection in real time PCR (qPCR)
  • Includes SYBR Green I for intercalator-based qPCR
  • Convenient 2X premix optimized for qPCR; simply add primers, template, and sterile distilled water to perform intercalator-based qPCR
  • Excellent for high-speed qPCR

Applications

  • Real Time PCR Quantitation of DNA using SYBR Green I and ROX Reference Dye

Components

RR420A (200 reactions (50 µL rxns); 1 x 5 mL)

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)1		1 mL × 5
ROX Reference Dye (50X conc.)2200 µL
ROX Reference Dye II (50X conc.)2200 µL

1: Contains TaKaRa Ex Taq HS, dNTP Mixture, Mg2+, Tli RNaseH and SYBR Green I

2: This component is to be used for analyses using a device that corrects fluorescent signals between wells such as the qPCR device by Applied Biosystems. Please use ROX Reference Dye for Applied Biosystems 7900HT/7300 Real-Time PCR System or StepOnePlus and ROX Reference Dye II for 7500 Real-Time PCR System or 7500 Fast Real-Time PCR System. ROX Reference Dye II is compatible with Agilent Mx3000P. This component is not required with Thermal Cycler Dice Real Time System II, Smart Cycler System or LightCycler.

RR420L (200 reactions (50 µL rxns); 1 x 5 mL)

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)*1 x 5 mL
ROX Reference Dye (50X conc.) 200 µL

RR420W (1000 reactions (50 µL rxns); 5 x 5 mL)

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)*5 x 5 mL
ROX Reference Dye (50X conc.) 1 mL

*Contains TaKaRa Ex Taq HS DNA Polymerase, dNTP mix, Mg2+ and SYBR Green I

RR42LR (200 reactions (50 µL rxns); 1 x 5 mL)

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)*1 x 5 mL

*Contains TaKaRa Ex Taq HS DNA Polymerase, dNTP Mix, Mg2+ and SYBR Green I and ROX Reference dye.

RR42WR (1000 reactions (50 µL rxns); 5 x 5 mL)

SYBR Premix Ex Taq (Tli RNaseH Plus) (2X conc.)*5 x 5 mL

*Contains TaKaRa Ex Taq HS DNA Polymerase, dNTP Mix, Mg2+ and SYBR Green I and ROX Reference dye.

Storage

4°C

Product is stable for up to 6 months when stored at 4°C. Protect this kit from light and avoid contamination. This kit is shipped in a dry ice-packed package. Store the kit protected from light at 4°C after receipt. For long-term storage, keep it at –80°C. (Do not store at –20°C). Store a thawed product at 4°C and use it within 6 months.

PCR Performance Test

Consistent amplification results were confirmed by SYBR Green I detection of PCR products in real time using a 300 bp lambda DNA template and a Smart Cycler real time PCR instrument.


 
 
Products
New Cat. # Product Package Size Price License # of Units Select
  RR420A SYBR® Premix Ex Taq™ (Tli RNase H Plus) 200 Rxns $284.00 License Statements
  RR420B SYBR® Premix Ex Taq™ (Tli RNase H Plus) 400 Rxns $555.00 License Statements
RR420L SYBR® Premix Ex Taq™ (Tli RNase H Plus), 1 x 5 mL 200 Rxns $284.00 License Statements
RR42LR SYBR® Premix Ex Taq™ (Tli RNase H Plus), 1 x 5 mL, ROX Plus 200 Rxns $284.00 License Statements
RR420W SYBR® Premix Ex Taq™ (Tli RNase H Plus), 5 x 5 mL 1,000 Rxns $1,136.00 License Statements
RR42WR SYBR® Premix Ex Taq™ (Tli RNase H Plus), 5 x 5 mL, ROX Plus 1,000 Rxns $1,136.00 License Statements
 

 


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