When synthesizing cDNA for real-time PCR, the PrimeScript RT reagent Kit (Perfect Real Time) allows maximal flexibility during reaction assembly. This reverse transcription reagent kit facilitates cDNA synthesis prior to analysis by real-time PCR. Designed for two-step real-time RT-PCR (also called qRT-PCR or RT-qPCR), the PrimeScript RT Reagent Kit (Perfect Real Time) contains all of the components needed for reverse transcription, including PrimeScript RTase, RNase Inhibitor, Random 6mers, Oligo dT Primer, dNTP Mixture and reaction buffer. Because the kit's primers and RTase are provided as separate components, there is greater flexibility in reaction assembly. If a master mix is preferred, consider using PrimeScript RT Master Mix (Perfect Real Time. Use this kit in combination with one of Takara Bio's qPCR premix products, such as SYBR Premix Ex Taq II (Tli RNase H Plus) (Cat.# RR820A), SYBR Premix Ex Taq (Tli RNase H Plus) (Cat.# RR420A), or Premix Ex Taq (Probe qPCR) (Cat.# RR390A), for SYBR Green or TaqMan probe-mediated detection.
The PrimeScript RT Reagent Kit (Perfect Real Time) includes PrimeScript Reverse Transcriptase for strong strand displacement activity and efficient synthesis of cDNA for real-time PCR. PrimeScript RTase is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to be RNase H Minus. PrimeScript RTase is also robust, versatile and can be used for reverse transcription of virtually any RNA template, including templates that have a high degree of secondary structure or are GC-rich. Because of the excellent extension capability of PrimeScript, cDNA synthesis reactions can be performed at 42°C, minimizing the risk of RNA degradation that often occurs at higher temperatures.