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Products >  PCR_Products >  Real_Time_qPCR_Kits >  RT-qPCR_Reagents >  PrimeScript_RT_Master_Mix_Perfect_Real_Time

Real-time RT-PCR Kit: PrimeScript RT Master Mix (Perfect Real Time)

To minimize sample handling time, look no further than this real-time RT-PCR kit. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. cDNA synthesis reactions are easy to assemble - just add RNA template and water. The kit contains a 5X master mix reagent that includes all reagents required for cDNA synthesis (PrimeScript RTase, RNase Inhibitor, random 6mers, Oligo dT Primer, dNTP Mixture, and reaction buffer). Because this real-time RT-PCR kit is powered by PrimeScript Reverse Transcriptase, it enables full-length cDNA synthesis for real-time RT-PCR (qPCR) in just 15 minutes. This kit is designed for two-step real-time RT-PCR. Use it upstream of a SYBR or TaqMan qPCR premix from Takara Bio, such as SYBR Premix Ex Taq II (Tli RNase H), SYBR Premix Ex Taq (Tli RNase H), or Premix Ex Taq (Probe qPCR).

This real-time RT-PCR kit includes PrimeScript Reverse Transcriptase, which has exceptionally strong strand displacement activity for optimal efficiency during cDNA synthesis. This recombinant RTase enzyme is robust, versatile and well-suited for applications requiring full-length cDNA synthesis. PrimeScript RTase enables reverse transcription of virtually any RNA template, including templates that are GC-rich and/or have high levels of secondary structure, making this real-time RT-PCR ideal for both routine applications and challenging reverse transcriptase reactions alike. PrimeScript RTase is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) enzyme and is RNase H Minus. PrimeScript Reverse Transcriptase is engineered to allow full-length cDNA synthesis reactions at lower temperatures (e.g., 42°C), decreasing the risk of RNA degradation that occurs during conventional reactions performed at higher temperatures.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Reverse transcription in just 15 minutes
  • Master Mix format reduces pipetting steps and the possibility of contamination
  • Works efficiently with GC-rich and.or higher-structured RNA templates
  • Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis

Applications

  • First-strand cDNA synthesis for real-time RT-PCR (qPCR)
  • Two-step real-time RT-PCR

Components

RR036A (200 reactions (10 µL rxns))

5X PrimeScript RT Master Mix (Perfect Real Time)*1 400 μL
RNase Free dH2O1 mL x 2
EASY Dilution (for Real Time PCR)*2 1 mL

RR036B (800 reactions (10 µL rxns))

5X PrimeScript RT Master Mix (Perfect Real Time)*1 400 μL x 4
RNase Free dH2O1 mL x 8
EASY Dilution (for Real Time PCR)*2 1 mL x 4

*1: Contains PrimeScript RTase, RNase Inhibitor, Oligo dT Primer, random 6mers, dNTP Mixture and reaction buffer (containing Mg2+).

*2: To be used when preparing serial dilutions of total RNA and cDNA. EASY Dilution makes it possible to obtain a precise dilution down to very low concentrations. This solution will not affect reverse-transcription and PCR reactivity. You may use the diluted solution directly in reverse-transcription and PCR. EASY Dilution (for Real Time PCR) (Cat. #9160) may also be purchased separately.

Note: Use EASY Dilution (for Real Time PCR) in combination with Takara Bio's Real-Time PCR kits. Compatibility with products from other manufacturers has not yet been verified.

Storage

-20°C

Choosing a PrimeScript Kit for Real-Time RT-PCR

If you want to....

Use this....

Make cDNA using a master mix that includes RTase and primers

PrimeScript RT Master Mix (Perfect Real Time) (Cat. # RR036A and RR036B)

Make cDNA using a kit that provides RTase and primers as separate components

PrimeScript RT Reagent Kit (Perfect Real Time) (Cat. # RR037A and RR037B)

Avoid genomic DNA contamination during cDNA synthesis and RT-qPCR

PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Cat. # RR047A and RR047B)

Perform 1-step real-time RT-PCR with SYBR Green

One-Step SYBR PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. # RR086A and RR086B)

Prepare cDNA for RT-qPCR directly from fewer than 1,000 cells

CellAmp Whole Transcriptome Amplification Kit (Real Time), Ver. 2 (Cat. # 3734)

References

1. Komori, H. et al. (2012) J. Biol. Chem. 287:30688-30700.

2. Liu, H. et al. (2012) Drug Metab. Dispos. 40:2109-2118.

3. Shimura, Y. et al. (2012) Plant Cell Physiol. 53:1255-1266.

4. Tani, H. et al. (2012) Genome Res. 22:947-956.

5. Yasuhara, K. et al. (2011) J. Appl. Physiol. 111:1142-1149.

6. Zhan, Y. et al. (2012) Cancer Prevention Res. 5:864-873. 

7. Zhang, X. et al. (2012) Proc. Natl. Acad. Sci. USA 109:21534-21539.




 
 
Products
Cat. # Product Contents Size Price Units Select
RR036A PrimeScript™ RT Master Mix (Perfect Real Time) 200 Rxns $425.00
RR036B PrimeScript™ RT Master Mix (Perfect Real Time) 800 Rxns $1,365.00
 

 

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RR420B SYBR® Premix Ex Taq™ (Tli RNase H Plus) 400 Rxns $566.00 License Statements
RR820A SYBR® Premix Ex Taq™ II (Tli RNase H Plus) 200 Rxns $290.00 License Statements
RR820B SYBR® Premix Ex Taq™ II (Tli RNase H Plus) 400 Rxns $566.00 License Statements
RR390A Premix Ex Taq™ (Probe qPCR) 200 Rxns $233.00 License Statements
RR390B Premix Ex Taq™ (Probe qPCR) 400 Rxns $463.00 License Statements
 

 

 
 

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