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Products >  PCR_Products >  Real_Time_qPCR_Kits >  RT-qPCR_Reagents >  BluePrint_RT_Reagent_Kit

BluePrint Reagent Kit for Real Time PCR

BluePrint RT Reagent Kit is designed to perform reverse transcription that has been optimized for real-time RT-PCR. It uses the new BluePrint RTase with excellent elongation ability for quick, efficient cDNA template synthesis for Real Time PCR. The protocol is simple and suitable for high throughput analysis.

This kit can be used in combination with qPCR reagents from most major suppliers including Takara's SYBR Premix Ex Taq (Perfect Real Time), SYBR Premix Ex Taq II (Perfect Real Time) or Premix Ex Taq (Perfect Real Time) for 2-step Real Time RT-PCR.

Optimized protocols are provided for use with either SYBR Green or TaqMan probe assay conditions.

At-A-Glance Documents Resources

Features

  • Optimized for qRT-PCR
  • Complete RT reaction in 15 minutes
  • Superior qPCR performance when used with any real time PCR enzyme

Components

5X BluePrint Buffer for Real Time1 400 µL
BluePrint Enzyme Mix 100 µL
Oligo dT Primer50 µM100 µL
Random 6 mers100 µM400 µL
RNase Free dH2O 2 x 1 mL
EASY Dilution for Real Time PCR2 1 mL

1: Contains dNTP Mixture and Mg2+.
2: Used as a dilution solution for creating dilution series of total RNA or cDNA. In contrast to dilution with water or TE, EASY Dilution facilitates accurate low concentration dilution. Diluted templates can be used as the template for reverse transcription or PCR reactions, because the EASY Dilution does not inhibit either reverse transcription or PCR enzyme activity. EASY Dilution (for Real Time PCR) is also available separately.

Note: EASY Dilution has been tested with Takara Bio’s real-time PCR reagents. Compatibility with products from other manufacturers has not yet been verified.


 
 
Products
Cat. # Product Package Size Price # of Units Select
RR737A BluePrint® Reagent Kit for Real Time PCR 200 Rxns $417.00
RR737B BluePrint® Reagent Kit for Real Time PCR 800 Rxns $1,427.00
 

 


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