The CellAmp Direct RNA Prep Kit RT-PCR (qPCR) is for preparation of templates used in one-step and two-step real-time RT-PCR utilizing a simple method of extraction directly from cultured cells in 96 well-plates or others. It is possible to prepare templates for real-time PCR from cultured cells in approximately 10 minutes.
Using this kit with a One-step real-time RT-PCR kit (i.e., One Step SYBR Ex Taq qRT-PCR kit) allows gene expression analysis to be performed in approximately 2 hours. Templates can be prepared directly from small quantities of cells which is useful for analysis of a gene expression profile and does not influence the high sensitivity of real-time RT-PCR. Genomic DNA can be removed efficiently using this kit. Therefore, it is powerful for expression analysis of genes, primers that can't be designed on both ends an exon junction, or where expression levels are low.