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Products >  PCR_Products >  Real_Time_qPCR_Kits >  RNA_Extraction_for_qPCR >  CellAmp_Direct_RNA_Prep_Kit_for_Real_Time_PCR

CellAmp Direct RNA Prep Kit for Real-Time PCR

The CellAmp Direct RNA Prep Kit RT-PCR (qPCR) is for preparation of templates used in one-step and two-step real-time RT-PCR utilizing a simple method of extraction directly from cultured cells in 96 well-plates or others. It is possible to prepare templates for real-time PCR from cultured cells in approximately 10 minutes.

Using this kit with a One-step real-time RT-PCR kit (i.e., One Step SYBR Ex Taq qRT-PCR kit) allows gene expression analysis to be performed in approximately 2 hours. Templates can be prepared directly from small quantities of cells which is useful for analysis of a gene expression profile and does not influence the high sensitivity of real-time RT-PCR. Genomic DNA can be removed efficiently using this kit. Therefore, it is powerful for expression analysis of genes, primers that can't be designed on both ends an exon junction, or where expression levels are low.

At-A-Glance Documents Images & Data Resources

Features

  • Quick and simple preparation of RNA (10 min) template from cultured cells
  • RNA can be used for One-Step or Two-Step qRT-PCR
  • Works with SYBR or probe detection
  • Gene Analysis in 2 hours
  • Easy, time-saving and flexible prep kit

Applications

  • Prepare RNA templates for qRT-PCR

Components

CellAmp Washing Buffer2 X 12.5 mL
CellAmp Processing Buffer10 mL
DNase I for Direct RNA Prep200 µL

Storage

–20°C. CellAmp Washing Buffer and CellAmp Processing Buffer can be stored at 4°C after thawing. Avoid contamination.




 
 
Products
Cat. # Product Package Size Price # of Units Select
3732 CellAmp™ Direct RNA Prep Kit for RT-PCR (Real Time) 200 Rxns $191.00
 

 



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