Real-Time One-Step RNA PCR Kit

Real-time PCR is a method in which the generation of PCR products may be monitored in real time. Real time RT-PCR (synthesis of cDNA from total RNA or mRNA using reverse transcriptase, and subsequent monitoring of the cDNA amplification products) is an essential tool for RNA analysis, since it allows analysis of even tiny amounts of RNA. With Takara's Real Time One Step RNA PCR Kit, the RT-PCR reaction is performed in a single tube and real-time monitoring of the amplification process can be performed using either SYBR Green I or TaqMan probes. Amplification is performed using TaKaRa Ex Taq, which provides the high specificity optimal for real time PCR. This kit is compatible with the SmartCycler (Cepheid) and other real time instruments.