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Products >  PCR_Products >  Real_Time_qPCR_Kits >  Probe_Detection >  Premix_Ex_Taq

Premix Ex Taq (Perfect Real Time)

Premix Ex Taq (Perfect Real Time) is a 2X premix specially designed for high speed, high sensitivity real time PCR using either TaqMan probes or SYBR Green I (not included). It consists of Takara's high fidelity-high performance TaKaRa Ex Taq Hot Start, and a newly formulated, real time buffer which provides superior specificity and increased amplification efficiency for real time PCR. Antibody-mediated hot start technology prevents nonspecific amplification due to mispriming and/or formation of primer dimers during the reaction assembly. The Taq antibody-polymerase complex is denatured in the first cycling step, releasing the polymerase and allowing DNA synthesis to proceed.

Additionally, two tubes of ROX Reference Dyes are supplied as separate components. ROX Dye is a convenient internal reference standard for use in normalizing signals due to non-PCR related fluorescence fluctuations that occur either between wells or over time.

Premix Ex Taq (Perfect Real Time) provides superior specificity, performance and amplification yield for real time PCR on all major real time instruments.

Compatible Cyclers:

SmartCycler, Light Cycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems 7300/7500, iCycler, MJ Opticon, Stratagene MX 3000.

A dynamic range of 7–8 orders of magnitude for SYBR Green I detection and 10 orders of magnitude with probe detection can be expected using Premix Ex Taq (Perfect Real Time), as well as sensitivity to 10 copies for SYBR Green I and 10 copies for probe detection.

At-A-Glance Documents Images & Data Resources

Features

  • High specificity and increased amplification efficiency for qPCR
  • Sensitive to 10 copies for SYBR Green I or probe detection
  • Use with either Taqman Probes or SYBR Green for detection
  • Compatible with most commonly used real time instruments

Applications

  • Real time PCR using either TaqMan Probes or SYBR Green I Detection

Components

RR039A

Premix Ex Taq Mix (2X conc.)* 5 x 1 mL
ROX Reference Dye (50X conc.) 200 µL
ROX Reference Dye II (50X conc.) 200 µL

*contains TaKaRa Ex Taq HS DNA Polymerase, dNTP mix, Mg2+

RR039W

SYBR Premix Ex Taq Mix (2X conc.)* 1 x 25 mL
ROX Reference Dye (50X conc.) 1 mL

*contains TaKaRa Ex Taq HS DNA Polymerase, dNTP mix, Mg2+
and SYBR Green

Storage

Stable at 4°C for 6 months when thawed. Protect from light and take care to not cause contamination. For long-term storage, store at –20°C.

This product is shipped at –20°C. Make the concentration uniform by gently inverting a tube before use.

PCR Performance

Consistent amplification results were confirmed by SYBR Green I detection of PCR products in real time PCR using a 300 bp lambda DNA template and a Smart Cycler real time PCR instrument. Inhibition of Ex Taq polymerase activity by the antibody was also confirmed to be more than 90% after an incubation reaction of 55°C for 10 minutes.

Note: The ROX Reference Dye/Dye II is supplied for performing normalization of fluorescent signal intensities within wells when used with real time PCR instruments which have this option. For ABI PRISM 7000/7700/7900HT and Applied Biosystems 7300 Real-Time PCR Systems, the use of ROX Reference Dye (50X) is recommended. For the Applied Biosystems 7500 Real-Time PCR System, use of ROX Reference Dye II is recommended. The use of ROX Reference Dye or Dye II is optional, and not required when using Smart Cycler and LightCycler real time instruments.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR039A Premix Ex Taq™ DNA Polymerase (Perfect Real Time) 200 Rxns $228.00 License Statements
RR039B Premix Ex Taq™ DNA Polymerase (Perfect Real Time) 400 Rxns $454.00 License Statements
RR039W Premix Ex Taq™ DNA Polymerase (Perfect Real Time), 25 mL 1,000 Rxns $911.00 License Statements
 

 


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