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Products >  PCR_Products >  Real_Time_qPCR_Kits >  Probe_Detection >  One_Step_Ex_Taq_qRT-PCR_Kit

One Step Ex Taq qRT-PCR Kit

The One Step Ex Taq qRT-PCR Kit is designed to perform single step real-time RT-qPCR using TaqMan probe1,2 for product detection. In this one step RT-PCR kit, the RT-PCR can be performed in a single tube, minimizing pipetting error and contamination risk. This TaqMan Real-Time PCR (qPCR) kit is suitable for detecting small amounts of RNA, such as viral RNA or RNA derived from rare or precious samples. Additionally, amplified products are monitored in real time, eliminating the need to verify results via electrophoresis following PCR.

Note: This product is also available in a sample size.

Applicable Real-Time PCR Instruments

Thermal Cycler Dice Real Time System, ABI PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System (Applied Biosystems), LightCycler (Roche Diagnostics), SmartCycler System/SmartCycler II System3 (Cepheid) and others.

1. TaqMan is a registered trademark of Roche Molecular Systems Inc.

2. Use the One-Step SYBR Ex Taq qRT-PCR Kit for one-step real-time RT-PCR using SYBR Green I.

3. SmartCycler is a registered trademark of Cepheid.

At-A-Glance Documents Images & Data

Features

  • Robust elongation capacity due to TaKaRa Ex Taq  
  • Antibody-mediated hot start polymerase eliminates primer-dimer and background issues 
  • Accurate quantification of low abundance samples such as RNA viruses
  • One step format reduces risk of pipetting errors and contamination

Applications

  • TaqMan Real-Time PCR (qPCR)

Components

2X One Step RT-PCR Buffer*3 x 840 µL
TaKaRa Ex Taq HS (5U/µL)100 µL
RT Enzyme Mix100 µL
RNase Free dH2O2 x 1.25 mL
ROX Reference Dye (50X conc.)**100 µL
ROX Reference Dye II (50X conc.)**100 µL

* Includes dNTP Mixture and Mg2+
** ROX Reference Dye/Dye II is used for normalization of signal intensity through background subtraction. For ABI PRISM 7000/7700/7900HT and Applied Biosystems 7300 Real-Time PCR System, the use of ROX Reference Dye (50X) is recommended. For Applied Biosystems 7500 Real-Time PCR System, the use of ROX Reference Dye II is recommended. The use of ROX Reference Dye or Dye II is optional; for example, Dye/Dye II is not required with use of the Thermal Cycler Dice Real Time System, SmartCycler or LightCycler real time instruments.

Storage

–20°C


 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR068A One Step Ex Taq™ qRT-PCR Kit 100 Rxns $292.00 License Statements
RR068B One Step Ex Taq™ qRT-PCR Kit 500 Rxns $1,312.00 License Statements
 

 


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