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Products >  PCR_Products >  Real_Time_qPCR_Kits >  Probe_Detection >  One_Step_Ex_Taq_qRT-PCR_Kit

One Step Ex Taq qRT-PCR Kit

One Step Ex Taq RT-PCR Kit (Perfect Real Time) is designed for real-time one-step RT-qPCR using TaqMan probe1,2 detection. In this kit, RT-PCR can be performed in a single tube, minimizing contamination and is suitable for detection of small amounts of RNA, such as a viral RNA. In addition, amplified products are monitored in real time, so there is no need to verify results through electrophoresis after PCR.

Note: This product is available in a sample size.

Applicable Real Time PCR Instruments

Thermal Cycler Dice Real Time System, ABI PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System (Applied Biosystems), LightCycler (Roche Diagnostics), Smart Cycler System/Smart Cycler II System3 (Cepheid) and others.

1. TaqMan is a registered trademark of Roche Molecular Systems Inc.

2. Use One Step SYBR Ex Taq RT-PCR Kit (Perfect Real Time) for real time one-step RT-PCR using SYBR Green I for detection.

3. Smart Cycler is a registered trademark of Cepheid.

At-A-Glance Documents

Features

  • New RT with strong elongation activity and high-performance TaKaRa Ex Taq Hot Start Version
  • Optimized buffer system and antibody mediated hot start
  • Accurate quantification of low abundance samples such as RNA viruses
  • Reduce pipetting errors and risk of contamination

Applications

  • One step qRT-qPCR using the TaqMan probes

Components

2X One Step RT-PCR Buffer*3 x 840 µL
TaKaRa Ex Taq HS (5U/µL)100 µL
RT Enzyme Mix100 µL
RNase Free dH2O2 x 1.25 mL
ROX Reference Dye (50X conc.)**100 µL
ROX Reference Dye II (50X conc.)**100 µL

* Includes dNTP Mixture and Mg2+.
** ROX Reference Dye/Dye II is used for normalization of intensity by background subtraction. For ABI PRISM 7000/7700/7900HT and Applied Biosystems 7300 Real-Time PCR System, the use of ROX Reference Dye (50X) is recommended. For Applied Biosystems 7500 Real-Time PCR System, the use of ROX Reference Dye II is recommended. The use of ROX Reference Dye or Dye II is optional. It is not required for use with Thermal Cycler Dice Real Time System, Smart Cycler or LightCycler real time instruments.

Storage

–20°C


 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR068A One Step Ex Taq™ qRT-PCR Kit 100 Rxns $286.00 License Statements
RR068B One Step Ex Taq™ qRT-PCR Kit 500 Rxns $1,286.00 License Statements
 

 


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