The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enables full-length cDNA synthesis from GC-rich and even highly structured RNA templates. Reverse transcription can be performed at 42°C, avoiding the risk of RNA degradation which may occur at higher temperatures The PrimeScript RT-PCR Kit generates high yield full-length cDNAs of up to 12 kb in length.
This two-step RT-PCR kit also includes TaKaRa Ex Taq HS for efficient, sensitive and specific amplification of generated cDNA. The combination of PrimeScript with TaKaRa Ex Taq HS results in reliable, efficient and reproducible RT-PCR from a wide variety of RNA templates.