During RT-PCR gene expression studies, accurate reverse transcription and PCR amplification are essential. The PrimeScript High Fidelity RT-PCR Kit includes all reagents needed for high fidelity amplification of cDNA from total RNA or mRNA and is designed for two-step endpoint RT-PCR. This kit includes PrimeScript High Fidelity Reverse Transcriptase, a highly accurate MMLV (Moloney Murine Leukemia Virus) reverse transcriptase, and PrimeSTAR Max DNA Polymerase, which has the highest fidelity of any commercially available PCR enzyme. PrimeSTAR Max DNA Polymerase is formulated as a premix and offers high amplification efficiency as well as compatibility with a wide range of template concentrations. As a result, this kit is well suited for any cDNA studies requiring high fidelity, such as RT-PCR gene expression studies (including gene splicing studies where alternative splicing products must be distinguished, or when discerning between closely related gene family members), cDNA library construction, and transcriptome analysis.
Fast and Accurate Quantification
PrimeScript Reverse Transcriptase, included in the PrimeScript High Fidelity RT-PCR Kit, efficiently synthesizes cDNA thanks to its exceptionally strong strand displacement activity. This RTase is robust, versatile and well-suited for applications requiring full-length cDNA, such as RT-PCR gene expression studies. PrimeScript High Fidelity Reverse Transcriptase can be used for reverse transcription of virtually any RNA template, including templates that are GC-rich and/or have high levels of secondary structure. PrimeScript RTase is a modified, recombinant RNase H Minus MMLV. Because of the excellent extension capability of this enzyme, cDNA synthesis can be performed at 42°C, decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.
First introduced in Japan, PrimeScript is now available to scientists worldwide. Over 3,700 peer-reviewed articles have been published in which PrimeScript High Fidelity Reverse Transcriptase is used for a variety of applications such as RT-PCR gene expression studies, transcriptome analyses, miRNA expression and regulation, molecular evolution, virology and microbiology.