Applications - Selective amplification of cDNA in the presence of genomic DNA
- One-Step cDNA synthesis and amplification
Components RR025A | | AMV Reverse Transcriptase XL (5 U/µL)* | 50 µL | | RNase inhibitor (40 units/µL) | 50 µL | | AMV-Optimized Taq (5 units/µL) | 50 µL | | 2X mRNA Selective PCR Buffer I** | 2 x 1.25 mL | | 2X mRNA Selective PCR Buffer II** | 2 x 1.25 mL | | MgCl2 (25 mM) | 500 µL | | dNTP/analog mixture*** | 250 µL | | Control F-1 primer (20 µM) | 10 µL | | Control R-1 primer (20 µM) | 10 µL | | Control Template | 10 µL | | RNase-Free dH2O | 1 mL | | Random 9-mers (50 µM) | 50 µL | | Oligo dT Primer (50 µM) | 50 µL |
*Manufactured by Life Sciences, Inc.
** We generally recommend the use of Buffer I. However, in cases of low yield or no amplification products due to low GC content in the target DNA, Buffer II is recommended.
*** dNTP analogues are mixed in with each 10 mM dNTP.
Storage –20°C
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