The RNA LA PCR Kit is designed to generate longer and more accurate RT-PCR products. The kit uses AMV RT XL as the reverse transcriptase, which allows for efficient synthesis of first-strand cDNA up to 12 kb. AMV RT XL also provides better thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures.
In addition, the kit uses LA Taq, which can synthesize products up to 40 kb in size with 6.5X better fidelity than Taq Polymerase. Both the reverse transcriptase and amplification reactions can be performed in a single tube. The components for 3'-RACE are also supplied.
