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Products >  PCR_Products >  RT-PCR-Two_Step >  AMV_Reverse_Transcriptase >  BcaBEST_RNA_PCR_Kit

BcaBEST RNA PCR Kit

The BcaBEST RNA PCR Kit is designed to perform both reverse transcription and DNA amplification in a single tube. The kit uses BcaBEST DNA Polymerase, which contains both DNA polymerase and reverse transcriptase for first strand cDNA synthesis. In contrast to standard reverse transcriptases, BcaBEST Polymerase has a temperature optimum of 65°C, which permits cDNA synthesis from difficult and highly structured RNA templates.

Bca-Optimized Taq Polymerase is used for second strand synthesis and subsequent PCR. This polymerase utilizes LA PCR technology for improved PCR length and accuracy. Random 9-mers, oligo-dT primers or a specific downstream primer that acts as an antisense primer in PCR can be used for cDNA synthesis.
At-A-Glance Documents Images & Data Resources

Features

  • Any RNA can be used as a template: especially useful for GC rich RNA or RNA with secondary structure
  • Can amplify messages up to 5 kb in length
  • BcaBEST DNA Polymerase is utilized to carry out transcription at an optimum reaction temperature of 65°C
  • Uses Bca-optimized Taq, an LA technology-based enzyme: specially optimized (for use with BcaBEST DNA Polymerase)
  • The kit includes random 9 mers or oligo-dT primers for first strand synthesis: a specific downstream primer should be prepared by the user
  • The whole procedure is carried out in a single tube: the reverse transcription is stopped via heat inactivation of the enzyme and the PCR reaction mix is added directly to the tube and amplified

Applications

  • Single-tube RT-PCR, using LA PCR technology for better PCR performance
  • Reverse transcription of RNAs with high amounts of secondary structure

Components

RR023A

BcaBEST DNA Polymerase (22 U/µL)50 µL
RNase Inhibitor (40 U/µL)25 µL
Random 9-mers (50 µM) 50 µL
Oligo dT Primer (50 µM)50 µL
RNase-Free dH2O (DEPC-treated) 1 mL
Bca-Optimized Taq (5 U/µL)25 µL
2X Bca 1st Strand Buffer2 x 1.25 mL
5X Bca 2nd Strand Buffer800 µL
dNTP Mixture (10 mM each)50 µL
MgSO4 (25 mM)500 µL
Control F-1 Primer (20 µmol/µL) 25 µL
Control R-1 Primer (20 µmol/µL) 25 µL
Positive Control RNA (1 µg/µL) 25 µL

Storage

–20°C


 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR023A BcaBEST™ RNA PCR Kit Ver. 1.1 100 Rxns $289.00 License Statements
RR023B BcaBEST™ RNA PCR Kit Ver. 1.1 200 Rxns $493.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
2630A AMV Reverse Transcriptase XL for RT-PCR 250 Units $186.00
4030 dNTP Mixture 3.2 mol EA/1.28 mL $56.00
 

 


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