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Products >  PCR_Products >  RT-PCR-One_Step_and_RACE >  MMLV_Reverse_Transcriptase >  PrimeScript_One_Step_RT-PCR_Kit

PrimeScript One Step RT-PCR Kit

This one-step RT-PCR kit allows efficient, specific cDNA synthesis and amplification in a single tube for endpoint analysis (e.g., gel electrophoresis). Workflow during 1-step RT-PCR is efficient because there is no need to add additional reagents prior to PCR. The PrimeScript One Step RT-PCR Kit includes PrimeScript RTase, an RNAse H minus MMLV reverse transcriptase that allows robust reverse transcription of almost any RNA template, including GC-rich or highly structured RNA. To enable cDNA amplification, this one-step RT-PCR kit also includes TaKaRa Ex Taq Hot Start (HS) DNA Polymerase, a thermostable polymerase that allows robust, specific amplification and is formulated for hot-start PCR. Thanks to the PrimeScript One Step Enzyme Mix, sample handling time and risk of contamination are minimized. The PrimeScript One Step RT-PCR Kit (Dye Plus) also includes electrophoresis loading dyes (blue and yellow) in the premix to further reduce sample handling steps and allow visual confirmation of gel loading.

Fast and Accurate Quantification

This one-step RT-PCR kit is powered by PrimeScript RTase, which has exceptionally strong strand displacement activity. PrimeScript RTase is robust, versatile and well-suited for applications requiring full-length cDNA synthesis. The PrimeScript RTase enzyme is a modified, recombinant MMLV reverse transcriptase and is RNase H Minus. First introduced in Japan, PrimeScript RTase is now available to scientists worldwide. Over 3,700 peer-reviewed articles have been published in which PrimeScript Reverse Transcriptase is used for a variety of applications such as gene expression, gene discovery, transcriptome analysis, miRNA expression and regulation, molecular evolution, virology and microbiology.

At-A-Glance Documents Images & Data Resources

Features

  • Efficient, fast RT-PCR in a single tube 
  • Works efficiently with GC-rich and highly structured RNA templates
  • TaKaRa Ex Taq HS allows specific amplification with minimal background
  • Quick and easy reaction preparation
  • Choose from one-step RT-PCR kits with or without gel loading buffer included in the reaction

Applications

  • One Step RT-PCR
  • Gene expression analysis by endpoint RT-PCR

Components

RR055A (50 reactions (50 µL))

PrimeScript One Step Enzyme Mix 100 µL
2X One Step Buffer 2 × 625 µL
Control F-1 Primer* (20 µM)20 µL
Control R-1 Primer** (20 µM)20 µL
Positive Control RNA (2 x 105 copies/µL)20 µL
RNase Free dH2O2 × 625 µL

* Upstream sense primer for Positive Control RNA
** Downstream anti-sense primer for Positive Control RNA

RR055B (200 reactions (50 µL))

This kit is comprised of 4 x RR055A.

RR057A (50 reactions (50 µL))

PrimeScript One Step Enzyme Mix 100 µL
2X One Step Buffer (Dye Plus)2 × 625 µL
Control F-1 Primer* (20 µM)20 µL
Control R-1 Primer** (20 µM)20 µL
Positive Control RNA (2 x 105 copies/µL)20 µL
RNase Free dH2O2 × 625 µL

* Upstream sense primer for Positive Control RNA
** Downstream anti-sense primer for Positive Control RNA

RR057B (200 reactions (50 µL))

This kit is comprised of 4 x RR057A.

Storage

–20°C

Choosing a PrimeScript Kit for Endpoint RT-PCR

If you want to....

Use this....

Prepare full-length cDNA using your own primers

PrimeScript Reverse Transcriptase (Cat. # 2680A and 2680B)

Prepare full-length cDNA using a kit that includes primers

PrimeScript First Strand cDNA Synthesis Kit (Cat. # 6110A and 6110B)

Perform 2-step RT-PCR with a kit that includes RTase, primers, and PCR enzyme

PrimeScript RT-PCR Kit (Cat. # RR014A and RR014B)

Perform cDNA cloning studies with extremely high fidelity

PrimeScript High Fidelity RT-PCR Kit (Cat. # R022A and R022B)

Perform 1-step PCR

PrimeScript  One-Step RT-PCR Kit, Ver. 2 (Cat. # RR055A and RR055B)

References

1. Ishii, A. et al. (2012) J. Gen. Virol. 93:2247-2251.

2. Kato, Y. et al. (2010) J. Biochem. 147:63-71.

3. Lu, P. et al. (2009) J. Pharmacol. Exp. Ther. 331:319-326.

4. Morio, F. et al. (2012) J. Antimicrob. Chemother. 67:2131-2138.

5. Yamayoshi, S. et al. (2013) J. Virol. 87:3335-3347.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR055A PrimeScript™ One Step RT-PCR Kit Ver.2 50 Rxns $291.00 License Statements
RR055B PrimeScript™ One Step RT-PCR Kit Ver.2 200 Rxns $930.00 License Statements
RR057A PrimeScript™ One Step RT-PCR Kit, Ver.2 (Dye Plus) 50 Rxns $291.00 License Statements
RR057B PrimeScript™ One Step RT-PCR Kit, Ver.2 (Dye Plus) 200 Rxns $930.00 License Statements
 

 


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