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Products >  PCR_Products >  RT-PCR-One_Step_and_RACE >  AMV_Reverse_Transcriptase >  mRNA_Selective_PCR_Kit

mRNA Selective PCR Kit

The mRNA Selective PCR Kit is a one-step RT-PCR kit designed to selectively amplify cDNA that has been reverse-transcribed from RNA. Such selective amplification of cDNA occurs because the kit selects the cDNA into which a dNTP analog has been incorporated during cDNA synthesis. The mRNA Selective PCR Kit enables successful cDNA synthesis from RNA by taking advantage of AMV (Avian Myeloblastosis Virus) Reverse Transcriptase XL in combination with AMV-Optimized Taq that was developed using LA (Long and Accurate) technology. Both the AMV Reverse Transcriptase XL and the AMV-Optimized Taq are capable of cooperatively functioning in a single tube to enable one step RT-PCR. This one-step RT-PCR kit includes all the reagents required for cDNA synthesis from RNA and selective amplification of cDNA.

At-A-Glance Documents Images & Data Resources

Applications

  • Selective amplification of cDNA in the presence of genomic and other DNA
  • One-step cDNA synthesis and amplification

Components

RR025A

AMV Reverse Transcriptase XL (5 U/µL)* 50 µL
RNase inhibitor (40 units/µL)50 µL
AMV-Optimized Taq (5 units/µL)50 µL
2X mRNA Selective PCR Buffer I** 2 x 1.25 mL
2X mRNA Selective PCR Buffer II** 2 x 1.25 mL
MgCl2 (25 mM)500 µL
dNTP/analog mixture*** 250 µL
Control F-1 primer (20 µM) 10 µL
Control R-1 primer (20 µM)10 µL
Control Template10 µL
RNase-Free dH2O 1 mL
Random 9-mers (50 µM) 50 µL
Oligo dT Primer (50 µM)50 µL

*Manufactured by Life Sciences, Inc.
** We generally recommend the use of Buffer I. However, in cases of low yield or no amplification products due to low GC content in the target DNA, Buffer II is recommended.
*** dNTP analogues are mixed in with each 10 mM dNTP.

Storage

–20°C

References

1. Lynas, C. et al. (1989) J. Pathol. 157:285-289.

2. Frohman, M.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:8998-9002.

3. Mallet, F. et al. (1995) BioTechniques 18:678-687.




 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR025A mRNA Selective PCR Kit Ver. 1.1 50 Rxns $383.00 License Statements
 

 

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