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    AMV Reverse Transcriptase
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RT-PCR (Two Step)


Products >  PCR_Products >  RT-PCR-One_Step_and_RACE >  AMV_Reverse_Transcriptase >  One-Step_RNA_PCR_Kit

One-Step RNA PCR Kit

Takara's One-Step RNA PCR Kit offers the convenience and time savings of performing reverse transcription and PCR in a single tube, without the need for changing buffers or adding reagents, minimizing the risk of contamination. AMV Reverse Transcriptase XL provides greater thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. The kit also contains AMV-Optimized Taq DNA Polymerase for efficient amplification of the cDNA synthesized by AMV Reverse Transcriptase XL.
At-A-Glance Documents Images & Data Resources

Applications

  • Reverse transcription (RT) PCR from RNA templates in a single tube, one step reaction
  • cDNA synthesis

Components

RR024A

AMV Reverse Transcriptase XL* (5 U/µL)50 µL
AMV-Optimized Taq DNA Polymerase (5 U/µL)50 µL
RNase Inhibitor (40 U/µL)60 µL
RNase-Free dH2O (DEPC-treated) 2 x 1 mL
10X One-Step RNA PCR Buffer250 µL
dNTP Mixture (10 mM each)250 µL
MgCl2 (25 mM)500 µL
Control F-1 Primer (20 pmol/µL) 25 µL
Control R-1 Primer (20 pmol/µL)25 µL
Positive control RNA (2 x 105 copies/µL)25 µL
*Manufactured by Life Science Co.

Storage

–20°C


 
 
Products
Cat. # Product Package Size Price License # of Units Select
RR024A One-Step RNA PCR Kit (AMV) 50 Rxns $321.00 License Statements
RR024B One-Step RNA PCR Kit (AMV) 100 Rxns $635.00 License Statements
 

 

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