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Products >  PCR_Products >  RT-PCR-One_Step_and_RACE >  AMV_Reverse_Transcriptase >  5_Prime_RACE_Core_Set

5' RACE Core Set

Reverse transcription of mRNA followed by PCR (RT-PCR) is a common cDNA cloning method; however, it can be difficult to obtain full-length cDNA using this method. Rapid amplification of cDNA ends (RACE) helps overcome this problem with traditional RT-PCR by using a gene-specific primer for reverse transcription followed by amplification with specifically designed oligonucleotide primers.

The 5' RACE Core Set takes advantage of inverse PCR to amplify and identify unknown 5' cDNA ends during cDNA cloning. The 5' RACE Kit also contains all the reagents required for reverse transcription, DNA-RNA hybrid degradation, circularization and subsequent amplification of the single-stranded cDNA.

Because the 5' RACE Core Set uses AMV Reverse Transcriptase XL to maximize first-strand cDNA length, it is recommended for use with TaKaRa Taq, TaKaRa Ex Taq, or TaKaRa LA Taq DNA Polymerase enzymes.

  At-A-Glance   Documents   Images & Data   Resources

Applications

  • RACE
  • cDNA cloning
  • Insert identification

Components

6122

AMV Reverse Transcriptase XL* (5 U/µL) 10 µL
RNase Inhibitor (40 U/µL)10 µL
10X RT Buffer (containing dNTP mix)15 µL
RNase H (60 U/µL) 10 µL
5X Hybrid RNA Degradation Buffer 150 µL
T4 RNA Ligase (40 U/µL) 10 µL
5X RNA (ssDNA) Ligation Buffer80 µL
40% PEG-6000 200 µL
RNase-Free dH2O (DEPC-treated)1 mL
Positive Control RT Primer (200 pmol/µL) 10 µL
Positive Control 1st Primer Pair (ea. 20 pmol/µL) 10 µL
Positive Control 2nd Primer Pair (ea. 20 pmol/µL) 10 µL
Positive Control RNA (10 ng/µL)10 µL
*Manufactured by Life Science Co.

Storage

–20°C

References

1. Ochman, H. et al. (1988) Genetics 120(3):621-623.



 
 
Products
Cat. # Product Contents Size Price Units Select
6122 5'-RACE Core Set 10 Rxns $315.00
 

 

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