Reverse transcription of mRNA followed by PCR (RT-PCR) is a common cDNA cloning method; however, it can be difficult to obtain full-length cDNA using this method. Rapid amplification of cDNA ends (RACE) helps overcome this problem with traditional RT-PCR by using a gene-specific primer for reverse transcription followed by amplification with specifically designed oligonucleotide primers.
The 5' RACE Core Set takes advantage of inverse PCR to amplify and identify unknown 5' cDNA ends during cDNA cloning. The 5' RACE Kit also contains all the reagents required for reverse transcription, DNA-RNA hybrid degradation, circularization and subsequent amplification of the single-stranded cDNA.
Because the 5' RACE Core Set uses AMV Reverse Transcriptase XL to maximize first-strand cDNA length, it is recommended for use with TaKaRa Taq, TaKaRa Ex Taq, or TaKaRa LA Taq DNA Polymerase enzymes.