The 5'-Full RACE Core Set uses inverse PCR to amplify an unknown 5'-end of a cDNA. The kit contains all the reagents needed for reverse transcription, degradation of the DNA-RNA hybrid, circularization of single-stranded cDNA and subsequent amplification.
Reverse transcription of mRNA followed by PCR is a common method for obtaining cDNA clones for expression analysis. However, it can be difficult to obtain full-length cDNA by this method. Rapid amplification of cDNA ends (RACE) helps overcome this problem by using a gene-specific primer for reverse transcription, followed by amplification with specially designed oligonucleotide primers.
The 5'-Full RACE Core Set uses AMV Reverse Transcriptase XL to maximize the length of the first-strand cDNA, and is recommended for use with Takara's Taq, Ex Taq, or LA Taq.
