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Products >  PCR_Products >  Pathogen_Detection_and_Screening_Kits >  Human_Pathogen_Detection >  Human_Papillomavirus_Typing_Set

Human Papillomavirus PCR Typing Set

PCR Human Papillomavirus Typing Set, a primer set for PCR, is designed to identify a broad range of human papillomavirus (HPV) types. This set utilizes two pairs of consensus primers designed for amplifying the sequence of HPV homologous region containing the E6 and E7 regions of HPV (228-268 bp). Of the HPVs, malignant HPV-16, -18, -33, -52b and -58 are amplified using the HPVpU-1M/HPVpU-2R primer pair; and benign HPV-6 and -11 are amplified using the HPVpU-31B/HPVpU-2R primer pair. With the digestion of PCR products by restriction enzymes (Acc I, Afa I, Ava I, Ava II and Bgl II, available as Enzyme Set A and subsequent agarose gel electrophoresis, HPV types can be identified. This primer set is optimized for use with Takara Taq, which can be purchased separately.

At-A-Glance Documents Images & Data Resources

Applications

  • Identify a broad range of human papillomavirus types

Components

6603

HPVpU-1M
(malignant forward primer, 25 pmol/µL)50 µL
HPVpU-31B
(benign forward primer, 25 pmol/µL)50 µL
HPVpU-2R
(common reverse primer, 25 pmol/µL 100 µL
Control Template HPV-TM
(malignant, 1 ng/µL) 50 µL
Control Template HPV-TB
(benign, 1 ng/µL)50 µL

Storage

–20°C

Control Template

This set includes both malignant and benign control template to verify the PCR amplified DNA fragment. Amplification using HPV-TM, HPV-TB as template and primer pairs of HPVpU-1M / HPVpU-2R, HPVpU-31B / HPVpU-2R respectively, yields approximately 60 bp of DNA fragments which differ from the HPV-amplified fragment.


 
 
Products
Cat. # Product Package Size Price # of Units Select
6603 PCR Human Papillomavirus Typing Set 50 Rxns $274.00
 

 

Recommended Products
Cat. # Product Package Size Price License # of Units Select
6604 Enzyme Set A 50 Rxns $274.00  
R001A TaKaRa Taq™ DNA Polymerase 250 Units $118.00 License Statements
 

 


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