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Products >  PCR_Products >  Pathogen_Detection_and_Screening_Kits >  Human_Pathogen_Detection >  Human_Papillomavirus_Typing_Set

Human Papillomavirus PCR Typing Set

Takara's Human Papillomavirus PCR Typing Set is designed for the identification of a broad range of human papillomavirus (HPV) types using HPV type-specific PCR. The Human Papillomavirus PCR Typing Set uses two pairs of consensus primers that are designed to amplify the E6 and E7 homologous regions of HPV (228-268 bp). Malignant HPV-16, -18, -33, -52b and -58 types are amplified using the HPVpU-1M/HPVpU-2R primer pair; benign HPV-6 and -11 types are amplified using the HPVpU-31B/HPVpU-2R primer pair. Digestion of PCR products by restriction enzymes Acc I, Afa I, Ava I, Ava II and Bgl II, available as Enzyme Set A, followed by agarose gel electrophoresis identifies the resident HPV types. The Human Papillomavirus PCR Typing Set is optimized for use with Takara Taq DNA Polymerase, which can be purchased separately.

At-A-Glance Documents Images & Data Resources

Applications

  • Identification of a broad range of HPV types
  • HPV type-specific PCR

Components

6603

HPVpU-1M
(malignant forward primer, 25 pmol/µL)50 µL
HPVpU-31B
(benign forward primer, 25 pmol/µL)50 µL
HPVpU-2R
(common reverse primer, 25 pmol/µL 100 µL
Control Template HPV-TM
(malignant, 1 ng/µL) 50 µL
Control Template HPV-TB
(benign, 1 ng/µL)50 µL

Storage

–20°C

Control Templates

This set includes both malignant and benign control templates for verification of PCR amplified DNA fragments. Amplification using HPV-TM and HPV-TB as the control templates with primer pairs HPVpU-1M / HPVpU-2R and HPVpU-31B / HPVpU-2R, respectively, yields approximately 60 bp DNA fragments. The control DNA fragments differ in size from those generated from HPV templates.

Note

For research use only.

References

1. Fujinaga, Y. et al. (1991) Journal of Gen. Virol. 72:1039-1044.



 
 
Products
Cat. # Product Package Size Price # of Units Select
6603 PCR Human Papillomavirus Typing Set 50 Rxns $282.00
 

 

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