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Products >  PCR_Products >  Pathogen_Detection_and_Screening_Kits >  Human_Pathogen_Detection >  Human_Papillomavirus_Detection_Set

Human Papillomavirus PCR Detection Set

The Human Papillomavirus PCR Detection Set offers a set of primers designed for human papillomavirus (HPV) detection of HPV 16, 18 and 33. These HPV types have all been detected in cervical carcinoma. This HPV PCR primer set allows simple and sensitive detection of HPV 16, 18 and 33 through amplification of the viral DNA. Included in this set are specific amplification primers of the E6 region of HPV 16, 18 and 33. Amplification product sizes are 140 bp for HPV 16 and 18 and 141 bp for HPV 33. HPV type-specific probes for subsequent hybridization are also provided with the Human Papillomavirus (HPV) Detection kit.

The Human Papillomavirus PCR Detection Set works very efficiently when used in conjunction with TaKaRa Taq DNA Polymerase.

At-A-Glance Documents Images & Data Resources

Applications

  • Human papillomavirus (HPV) detection
  • HPV PCR 

Components

6602

HPVpF
(common forward primer, 25 pmol/µL)100 µL
HPVp16R
(HPV 16 reverse primer, 25 pmol/µL)50 µL
HPVp18R
(HPV 18 reverse primer, 25 pmol/µL50 µL
HPVp33R

(HPV 33 reverse primer, 25 pmol/µL)

50 µL
HPVb16
(HPV 16 probe, 25 pmol/µL)10 µL
HPVb18
(HPV 18 probe, 25 pmol/µL)10 µL
HPVb33
(HPV 33 probe, 25 pmol/µL)10 µL
Control Template
HPVT16 (HPV16, 1 ng/µL)50 µL
HPVT18 (HPV18, 1 ng/µL)50 µL
HPVT33 (HPV33, 1 ng/µL)50 µL

Storage

–20°C

Control Templates

The Human Papillomavirus PCR Detection Set includes three control templates to verify the presence of PCR amplified DNA fragments. Amplification reactions using control templates HPVT16, HPVT18 and HPVT33 and primer pairs HPVpF/HPVp16R, HPVpF/HPVp18R and HPVpF/HPVp33R, respectively, yield 70 bp DNA fragments. Because the control templates contain sequences complementary to the probes provided in the Human Papillomavirus PCR Detection Set, they can be used as positive controls for each HPV type via dot hybridization.

Note

This product is for research use only.

References

1. Shimada, M. et al. (1990) Jpn. J. Cancer Res. 81:1-5.



 
 
Products
Cat. # Product Package Size Price # of Units Select
6602 PCR Human Papillomavirus Detection Set 50 Rxns $282.00
 

 

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