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Products >  PCR_Products >  Pathogen_Detection_and_Screening_Kits >  Human_Pathogen_Detection >  Human_Papillomavirus_Detection_Set

Human Papillomavirus PCR Detection Set

PCR Human Papillomavirus Detection Set is a primer set designed to detect the presence of human papillomavirus (HPV) 16, 18 and 33 which have been found in cervical carcinoma. This primer set allows simple and sensitive detection of HPV 16, 18 or 33 by specific amplification of their DNA using PCR. Included in this set are the specific primers for amplification of the sequence containing E6 region of HPV 16, 18 and 33 (140 bp for HPV 16, 18 and 141 bp for HPV 33) and the HPV type-specific probes for the subsequent hybridization. This primer set will work most efficiently when used in conjunction with TaKaRa Taq.

At-A-Glance Documents Resources

Applications

  • Detection of presence of human papillomavirus

Components

6602

HPVpF
(common forward primer, 25 pmol/µL)100 µL
HPVp16R
(HPV 16 reverse primer, 25 pmol/µL)50 µL
HPVp18R
(HPV 18 reverse primer, 25 pmol/µL50 µL
HPVp33R

(HPV 33 reverse primer, 25 pmol/µL)

50 µL
HPVb16
(HPV 16 probe, 25 pmol/µL)10 µL
HPVb18
(HPV 18 probe, 25 pmol/µL)10 µL
HPVb33
(HPV 33 probe, 25 pmol/µL)10 µL
Control Template
HPVT16 (HPV16, 1 ng/µL)50 µL
HPVT18 (HPV18, 1 ng/µL)50 µL
HPVT33 (HPV33, 1 ng/µL)50 µL

Storage

–20°C

Control Template

This set includes a Control Template to verify the PCR amplified DNA fragments. Amplification using HPVT16, HPVT18, HPVT33 as template and primer pairs of HPVpF/HPVp16R, HPVpF/HPVp18R, HPVpF/HPVp33R respectively, yields 70 bp of DNA fragments. As this Control Template contains the sequences complementary to the probes provided in the set, it can be used as positive control for each type of HPV in dot hybridization.




 
 
Products
Cat. # Product Package Size Price # of Units Select
6602 PCR Human Papillomavirus Detection Set 50 Rxns $274.00
 

 

Recommended Products
Cat. # Product Package Size Price License # of Units Select
R001A TaKaRa Taq™ DNA Polymerase 250 Units $118.00 License Statements
 

 


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