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Products >  PCR_Products >  PCR_Enzymes_and_Kits >  PCR_Selection_Guide

PCR Selection Guide

High Yield

Application Mammalian Genotyping Library Construction Routine PCR Low Copy Number and/or Dirty Samples
Recommended product Titanium Taq Advantage 2 EmeraldAmp MAX HS PCR Master Mix TaKaRa Ex Taq DNA Polymerase
Choose for: Sensitive and robust Long PCR and enhanced fidelity Streamlined workflow Higher fidelity and yield
Amplicon size
gDNA <2 kb <6 kb <15 kb <20 kb
Plasmid/lambda <2 kb <18.5 kb NA <30 kb
cDNA <4 kb <8.5 kb NA NA
Enzyme properties
5'–3' exonuclease activity Yes Yes
3'–5' exonuclease activity Yes Yes Yes
T/A overhangs or Blunt T/A T/A T/A T/A
Format/Features
Premix Yes Available
Eco-Friendly format Available Available
PCR Kit with control template and primers available Yes Yes
Dye added Yes
Hot Start Yes Yes Yes Available
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High Fidelity

Application In-Fusion Cloning Long PCR, sequencing, cloning Cloning and expression, DNA sequencing
Recommended product CloneAmp HiFi PrimeSTAR GXL PrimeSTAR Max
Choose for: In-Fusion Cloning Excellent all-around performance on a variety of templates Highest fidelity
Amplicon size
gDNA <6 kb (human),
<10 kb (E. coli)
<30 kb <6 kb
Plasmid/lambda <15 kb <40 kb <15 kb
cDNA <6 kb <13.5 kb <10 kb
Enzyme properties
5'–3' exonuclease activity
3'–5' exonuclease activity Yes Yes Yes
T/A overhangs or Blunt Blunt Blunt Blunt
Fidelity (sequencing) 29X Taq (GC-rich target) 6.5X Taq (GC-rich target) 29X Taq (GC-rich target)
Speed 5 sec/kb 10 sec/kb (rapid protocol) 5 sec/kb
GC-Rich targets Up to 73%
Format/Features
Premix Yes Yes
Hot Start Yes Yes Yes
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Lyophilized

Application High throughput genotyping High throughput genotyping
Recommended product High Yield PCR EcoDry Premix High Fidelity PCR EcoDry Premix
Choose for: High yield and easy-to-use Long PCR and enhanced fidelity
Amplicon size
gDNA <2 kb <6 kb
Plasmid/lambda <2 kb <18.5 kb
cDNA <4 kb <8.5 kb
Enzyme properties
5'–3' exonuclease activity
3'–5' exonuclease activity Yes
T/A overhangs or Blunt T/A T/A
Fidelity (Kunkel) 1X Taq 3X Taq
Format/Features
Premix Yes Yes
Eco-Friendly lyophilized format Yes Yes
Dye added
Hot Start Yes Yes
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Long PCR

Application Long PCR, sequencing, cloning Long PCR, GC-rich, cloning, sequencing Long PCR, sequencing, cloning
Recommended product TaKaRa LA Taq DNA Polymerase TaKaRa LA Taq DNA Polymerase with GC Buffers PrimeSTAR GXL
Choose for: Maximum length High fidelity Excellent all-around performance on a variety of templates
Amplicon size
gDNA <30 kb <17.5 kb <30 kb
Plasmid/lambda <48 kb <35 kb <40 kb
Enzyme properties
5'–3' exonuclease activity Yes Yes
3'–5' exonuclease activity Yes Yes Yes
T/A overhangs or Blunt T/A T/A Blunt
Fidelity (Kunkel) 6.5X Taq (Standard %GC target) 6.5X Taq (Standard %GC target)
Fidelity (Sequencing) 6.5X Taq (GC-rich target)
Speed 60 sec/kb 60 sec/kb 10 sec/kb (rapid protocol)
GC-Rich targets Up to 67% Up to 74% Up to 73%
Format/Features
Premix Available
Hot Start Available Yes
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GC-Rich

Application Robust genotyping Long PCR, sequencing, cloning Amplification prior to NGS
Recommended product Advantage GC 2 Polymerase Mix TaKaRa LA Taq DNA Polymerase with GC Buffers PrimeSTAR GXL
Choose for: High % GC Long PCR with GC-rich templates Highest fidelity
Amplicon size
gDNA <3.5 kb <17.5 kb <30 kb
Plasmid/lambda <6 kb <35 kb <40 kb
cDNA <2 kb NA <13.5 kb
Enzyme properties
5'–3' exonuclease activity Yes
3'–5' exonuclease activity Yes Yes Yes
T/A overhangs or Blunt T/A T/A Blunt
Fidelity (Kunkel) 3X Taq (Standard %GC target) 6.5X Taq (Standard %GC target)
Fidelity (Sequencing) 6.5X Taq (GC-rich target)
Speed 60 sec/kb 60 sec/kb 10 sec/kb (rapid protocol)
GC-Rich targets Up to 90% Up to 74% 73%
Format/Features
PCR Kit with control template and primers Available Available
Hot Start Yes
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Direct PCR

Application Mouse tissues Mouse tissues Compatible with tough tissue Direct genotyping on FFPE samples Direct genotyping on FTA Cards
Recommended product Terra PCR Direct Polymerase Mix Terra PCR Direct Red Dye Premix Terra PCR Direct Genotyping Kit Terra PCR Direct FFPE Kit Terra PCR Direct Card Kit
Choose for: Genotyping Direct genotyping and streamlined workflow Genotyping and adaptable workflow Genotyping with fixed samples Genotyping with archived samples
Amplicon size
gDNA <2 kb <2 kb <2 kb <2 kb <2 kb
Enzyme properties
T/A overhangs or Blunt T/A T/A T/A T/A T/A
GC-Rich targets 70% 70% 70% 70% 70%
Format/Features
Premix Yes
Dye added Yes Loading dye included separately
Hot Start Yes Yes Yes Yes Yes
Direct PCR Yes Yes Yes Yes Yes
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Fast PCR

Application Cloning, sequencing Colony PCR High throughput
Recommended product PrimeSTAR Max SapphireAmp Fast PCR Master Mix SpeedSTAR HS DNA Polymerase
Choose for: Fast, convenient, and highest fidelity Fast routine PCR Fast moderate fidelity PCR
Amplicon size
gDNA <6 kb <6 kb <20 kb
Plasmid/lambda <15 kb <5 kb <20 kb
cDNA <6 kb NA NA
Enzyme properties
5'–3' exonuclease activity Yes Yes
3'–5' exonuclease activity Yes Yes Yes
T/A overhangs or Blunt Blunt T/A T/A
Speed 5 sec/kb 10 sec/kb 10 sec/kb
Format/Features
Premix Yes Yes
Dye added Yes
Hot Start Yes Yes Yes
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General Purpose

Application General applications, multiplexing Genotyping High yield genotyping
Recommended product TaKaRa Taq DNA Polymerase EmeraldAmp GT PCR Master Mix EmeraldAmp MAX PCR Master Mix
Choose for: Routine use Value choice Streamlined workflow
Amplicon size
gDNA <3 kb <4 kb <15 kb
Plasmid/lambda <12 kb NA NA
cDNA NA NA NA
Enzyme properties
5'–3' exonuclease activity Yes Yes Yes (weak)
3'–5' exonuclease activity Yes
T/A overhangs or Blunt T/A T/A T/A
Format/Features
Premix Available Yes Yes
Dye added Yes Yes
Hot Start Available Available
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